临床与病理杂志2024，Vol.44Issue(10)：1343-1351,9.DOI:10.11817/j.issn.2095-6959.2024.240744
基于生物信息学探讨TAP1在胃腺癌中的表达及其意义
Expression and significance of TAP1 in gastric adenocarcinoma based on bioinformatics
摘要
Abstract
Objective:The transporter associated with antigen processing 1(TAP1)is closely related to autoinflammatory diseases,autoinflammatory disease,and cancers,but its relationship with gastric adenocarcinoma remains unclear.This study aims to explore the expression of TAP1 in gastric adenocarcinoma and its significance. Methods:The expression of TAP1 in gastric adenocarcinoma was detected using the Genotype-Tissue Expression(GTEx)and The Cancer Genome Atlas(TCGA)databases and immunohistochemistry.The correlation between TAP1 expression and pathological parameters was analyzed,and receiver operating characteristic(ROC)curve analysis was performed using the R software pROC package.The correlation between TAP1 expression and the prognosis of GAC was assessed through the Kaplan-Meier Plotter online analysis tool.The correlation between TAP1 expression and immune cell infiltration was analyzed using the Tumor Immune Estimation Resource(TIMER).Additionally,the top 100 genes co-expressed with TAP1 in gastric adenocarcinoma were identified through the Gene Expression Profiling Interactive Analysis(GEPIA)database,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis. Results:Compared to normal tissue,TAP1 protein and mRNA expression levels were significantly upregulated in gastric adenocarcinoma tissues(both P<0.05).The diagnostic ROC curve showed an area under the curve(AUC)of 0.826.TAP1 expression levels were correlated with tumor grading(P<0.01)but were not related to pathological staging or age(both P>0.05).Kaplan-Meier survival curves showed that patients with high TAP1 expression had significantly longer overall survival than those with low TAP1 expression(both P<0.05).Enrichment analysis revealed that TAP1 expression is closely related to T cell receptor binding,major histocompatibility complex(MHC)protein complex formation,and antigen processing and presentation.The expression of immune cell infiltration,such as CD4+T lymphocytes,was positively correlated with TAP1 expression(all P<0.05). Conclusion:TAP1 is highly expressed in gastric adenocarcinoma,with good diagnostic efficacy and is associated with better prognosis.TAP1 may be involved in biological processes such as T cell receptor binding,antigen processing and presentation,and is highly correlated with immune cell infiltration.关键词
胃腺癌/预后标志物/免疫浸润/抗原处理相关转运蛋白1/生物信息学/基因富集分析Key words
gastric adenocarcinoma/prognostic biomarker/immune infiltration/transporter associated with antigen processing 1/bioinformatics/gene enrichment analysis引用本文复制引用
汪巧,李光耀,戴敏..基于生物信息学探讨TAP1在胃腺癌中的表达及其意义[J].临床与病理杂志,2024,44(10):1343-1351,9.基金项目
安徽省卫生健康委员会省青年财政支持项目(AHWJ2023A30159).This work was supported by the Anhui Provincial Health Commission Provincial for Youth Programs,China(AHWJ2023A30159). （AHWJ2023A30159）
