林业与生态科学2025，Vol.40Issue(1)：18-25,8.DOI:10.13320/j.cnki.hjfor.2025.0003
光肩星天牛雌虫复眼响应光信号差异基因分析及其生物防治相关基因筛选
Differential gene expression analysis in the compound eyes of female Anoplophora glabripennis in response to light signals and screening of genes related to biological control
摘要
Abstract
The research aims to investigate the gene response of Anoplophora glabripennis compound eyes to light signals and explore the genes related to biological control of Anoplo-phora glabripennis,providing theoretical basis for the control of this beetle.Comparative transcriptome analysis method was used to analyze the differential gene expression of 4 treat-ments:light tretment to ventral area(LV),light tretment to dorsal area(LD),dark treat-ment to ventral area(DV)and dark treatment to dorsal area(DD).The results showed that:(1)Transcriptome sequencing was performed on 12 samples,and 16 200 genes were obtained;A total of 4 166 differential expression genes were found,including 649,2 588,1693 and 3 522 d in DV vs DD,LV_vs_LD,LV vs_DV,and LV_vs_DD,respectively.(2)GO term analysis showed that differential genes were mainly enriched in peptide biosynthe-sis,translation,amide biosynthesis,peptide metabolism,and cellular macromolecular bio-synthesis.(3)KEGG pathway analysis showed that differential genes were mainly concen-trated in ribosome,oxidative phosphorylation and thermogenesis.(4)Furthermore,106 genes related to biological control were found,including 31 olfaction-related genes,58 molt-ing-related genes,6 chitin synthesis-related genes and 11 lignocellulose-related genes.The re-sult provides a theoretical basis for using RNA interference to control A.glabripennis.关键词
光肩星天牛/复眼/转录组/光信号/生物防治Key words
Anoplophora glabripennis/compound eye/transcriptome/light signal/biological control分类
农业科技引用本文复制引用
李滕飞,陈鹏,黄晨,王志刚,吕飞..光肩星天牛雌虫复眼响应光信号差异基因分析及其生物防治相关基因筛选[J].林业与生态科学,2025,40(1):18-25,8.基金项目
国家自然科学基金项目(32171800). （32171800）
