黑龙江动物繁殖2025，Vol.33Issue(1)：24-30,36,8.DOI:10.19848/j.cnki.ISSN1005-2739.2025.02.0005
精子选择方法对牛精子质量及体外受精效果的影响
Effects of sperm selection methods on bovine sperm quality and in vitro fertilization outcomes
摘要
Abstract
To explore the effects of different sperm selection methods on sperm quality and embryo development after in vitro fertilization(IVF),bovine frozen semen was treated with the TALP liquid direct centrifugation method(control group),TALP Percoll density gradient centrifugation(group 1)and TALP PureSperm density gradient centrifugation(group 2)to evaluate the sperm motility,motility parameters(forward motile sperm ratio,average path velocity(VAP),linear velocity(VSL),curvilinear velocity(VCL),straightness(STR),head lateral displacement amplitude(ALH),plasma membrane integrity,acrosomal membrane integrity,mitochondrial membrane potential level,reactive oxygen species(ROS),concentration,DNA integrity,capacitated sperm rate,acrosome reaction sperm rate,tyrosine phosphorylation level and compare the embryonic development after IVF(cleavage rate,blastocyst rate)in different treatment groups.The results showed that the sperm motility,the proportion of forward motile sperm,the percentage of forward motile sperm and the percentage of forward motile sperm,VAP,VSL,VCL,STR,ALH,the sperm plasma membrane integrity rate and acrosome reaction sperm rate were significantly higher than those in the control group(P<0.05).The sperm DNA integrity rate was higher than that in the control group(P>0.05),and the sperm ROS content was significantly lower than that in the control group(P<0.05).Except for ROS content,other indicators in group 1 were higher than those in group 2,in which the difference in sperm motility was significant(P<0.05),while no significant difference in other indicators between the two groups(P>0.05).The sperm acrosomal membrane integrity rate and sperm tyrosine phosphorylation level in group 1 and in control group were significantly higher than those in group 2(P<0.05),while no significant difference between group 1 and control group(P>0.05).Sperm mitochondrial membrane potential and capacitated sperm rate in group 1 were significantly higher than those in control group and in group 2(P<0.05);The cleavage rate and blastocyst rate in group 1 were significantly higher than those in the control group(P<0.05),no significant difference with those in group 2(P>0.05).It could be concluded that the quality of bovine frozen semen selected by TALP Percoll DGC method could be preferred to achieve a better effect of embryo development after in vitro fertilization and used for bovine IVF sperm selection.关键词
牛/体外受精/精子选择/精子获能/Percoll/PureSperm/胚胎发育Key words
bovine/IVF/sperm selection/sperm capacitation/Percoll/PureSperm/embryo development分类
农业科技引用本文复制引用
张楠,郭桃,韩僖彤,张建军,张家新..精子选择方法对牛精子质量及体外受精效果的影响[J].黑龙江动物繁殖,2025,33(1):24-30,36,8.基金项目
内蒙古自治区揭榜挂帅项目(2022JBGS0025) （2022JBGS0025）
