华南农业大学学报2025，Vol.46Issue(2)：164-174,11.DOI:10.7671/j.issn.1001-411X.202402018
OsCdc48与Pik1-H4的互作机制及对稻瘟病抗性的调控
Interaction mechanism between OsCdc48 and Pik1-H4,and its regulation on rice blast disease resistance
摘要
Abstract
[Objective]To investigate the interaction mechanism between Pik1-H4 and cell division cycle protein OsCdc48,clarify its role in rice blast disease resistance.[Method]Firstly,the interactions between Pik1-H4 and OsCdc48 were verified using yeast two-hybrid and luciferase complementation assays.Then,the expression patterns of OsCdc48 after infection of Magnaporthe oryzae and its tissue-specific expression were analyzed by RT-qPCR.Next,the determination of OsCdc48's sequence conservation,protein domains,phylogenetic relationships,protein 3D structure prediction and subcellular localization were performed.Finally,OsCdc48 mutant was created using CRISPR/Cas9 technology,the resistance identification of transgenic mutant to rice blast disease and expression analysis of pathogenesis-related genes were also conducted.[Result]The interactions between Pik1-H4 and OsCdc48 were confirmed,and OsCdc48 was induced by rice blast fungus infection.OsCdc48 was expressed in all tissues and was localized to the nucleus and cytoplasm.The sequence of OsCdc48 was conserved across different species,with the closest phylogenetic relationship to maize and sorghum,and it might form homologous hexamers.The OsCdc48 loss-of-function mutant ko-oscdc48 up-regulated the expression of disease-related genes and enhanced the resistance to rice blast disease.[Conclusion]This study lays a theoretical basis for further elucidating the mechanism of OsCdc48 and NLR protein Pik1-H4 regulating blast disease resistance and rice disease-resistant breeding.关键词
水稻/稻瘟病/稻瘟病菌/Pik1-H4/OsCdc48Key words
Oryza sativa L./Rice blast disease/Magnaporthe oryzae/Pik1-H4/OsCdc48分类
生物科学引用本文复制引用
黄其伟,张丽娜,王加峰,陈淳..OsCdc48与Pik1-H4的互作机制及对稻瘟病抗性的调控[J].华南农业大学学报,2025,46(2):164-174,11.基金项目
广东省科技计划(2023B0202010003) （2023B0202010003）
广东省自然科学基金(2023A1515012101) （2023A1515012101）
