华南农业大学学报2025，Vol.46Issue(2)：175-185,11.DOI:10.7671/j.issn.1001-411X.202404046
希金斯炭疽菌自噬相关基因ChAtg3的功能研究
Study on the functions of the autophagy related gene ChAtg3 in Colletotrichum higginsianum
摘要
Abstract
[Objective]To study the role of ChAtg3,an autophagy-related gene in Colletotrichum higginsianum.[Method]The sequence,phylogeny and expression pattern of ChAtg3 were analyzed,and the expression changes of ChAtg3 were observed in Arabidopsis thaliana after infection with C.higginsianum.An Agrobacterium tumefaciens-mediated genetic transformation system was employed to perform specific gene knockouts and their subsequent complementation.[Result]The expression changes of ChAtg3 were observed after C.higginsianum infected A.thaliana for 8 to 40 h.The mutant ΔChAtg3,created through targeted ChAtg3 gene knockout,exhibited impaired autophagy,with markedly reduced melanin production,conidiation,appressorium formation,and pathogenic potential,although mycelial growth and conidial germination rate had no significant changes compared with wild type.Gene complementation in the CΔChAtg3 strain effectively reinstated its biological phenotype and pathogenicity.[Conclusion]The results reveal that ChAtg3 plays a key role in autophagy,conidiation,and appressorium formation of C.higginsianum,confirming it as a critical determinant of pathogenic capacity of C.higginsianum.关键词
希金斯炭疽菌/自噬/ChAtg3基因/基因功能分析/致病力Key words
Colletotrichum higginsianum/Autophagy/ChAtg3gene/Functional analysis of gene/Pathogenicity分类
植物保护学引用本文复制引用
段灵涛,何九卿,王莉,陈炜伦,祝一鸣,周而勋..希金斯炭疽菌自噬相关基因ChAtg3的功能研究[J].华南农业大学学报,2025,46(2):175-185,11.基金项目
广东省自然科学基金(2021A1515011166) （2021A1515011166）
国家重点研发计划(2017YFD0200900) （2017YFD0200900）
