华南农业大学学报2025，Vol.46Issue(2)：186-193,8.DOI:10.7671/j.issn.1001-411X.202406003
荔枝褐斑病病原鉴定及生防菌筛选
Pathogen identification of lychee brown spot and screening of its biocontrol agents
摘要
Abstract
[Objective]To identify the pathogen species of newly occurring lychee brown spot,determine the biological characteristics of the pathogen,and screen biocontrol agents,so as to provide a basis for understanding the occurrence patterns of lychee brown spot and green disease control.[Method]In October,2021,leaves with severe brown spot on young litchi trees were collected.A strain of GZ1 was obtained from diseased leaves and its pathogenicity was determined based on Koch's postulates.Morphological analysis and a phylogenetic tree based on sequences of internal transcribed spacer(ITS),β-tubulin gene(Tub2),and RNA polymerase Ⅱ second largest subunit gene(rpb2)were performed to clarify the taxonomic status of this pathogen.The biological characteristics of pathogen GZ1 were determined under various culture conditions including multiple carbon sources,temperatures,and pH.Antagonistic effects of two biocontrol agents,Bacillus subtilis XX and B.velezensis BS against GZ1 were evaluated using the dual culture method and hyphal growth rate method.[Result]The strain GZ1 was confirmed as the causal agent of lychee brown spot,and it was identified as Exserohilum rostratum(Drechsler)K.J.Leonard&Suggs.The optimal temperature and pH for its growth were 28℃and 7.It could utilize various carbon sources,with the fastest hyphal growth observed in the presence of sucrose.The biocontrol agents B.subtilis XX and B.velezensis BS exhibited significant inhibitory effects on the growth of pathogen GZ1.[Conclusion]This study reports that E.rostratum is the causal agent of lychee brown spot,thereby identifies a new lychee disease.The study can provide a basis for scientific control of lychee disease caused by E.rostratum.关键词
荔枝褐斑病/嘴突凸脐蠕孢/病原鉴定/生防菌Key words
Lychee brown spot/Exserohilum rostratum/Pathogen identification/Biocontrol agent分类
植物保护学引用本文复制引用
丁晓,宋雨,习平根,李敏慧,姜子德,孔广辉..荔枝褐斑病病原鉴定及生防菌筛选[J].华南农业大学学报,2025,46(2):186-193,8.基金项目
广东省自然科学基金(2023A1515030267) （2023A1515030267）
广州市科技计划(202201010410) （202201010410）
国家荔枝龙眼产业技术体系建设项目(CARS-32) （CARS-32）
