华南农业大学学报2025，Vol.46Issue(2)：194-202,9.DOI:10.7671/j.issn.1001-411X.202402020
红树林沉积物生防芽孢杆菌资源筛选及防效评价
Screening of biocontrol Bacillus strains in mangrove sediments and evaluation of efficacy
摘要
Abstract
[Objective]Utilizing the rich microbial resources in mangrove sediments,we screen for Bacillus strains with broad-spectrum antagonistic activity to provide a support for the development of highly efficient and green biological control agents to combat important agricultural diseases such as fire blight.[Method]Using the thermotolerant characteristic of Bacillus,we specifically screened Bacillus strains with antagonistic activity against Erwinia amylovora,the causal agent of fire blight.We also used the plate confrontation method to detect the antagonistic activity of these Bacillus strains against Xanthomonas oryzae pv.oryzae,Ralstonia solanacearum,Colletotrichum gloeosporioides and Fusarium graminearum,then focused on the control effect of these Bacillus strains against fire blight and strawberry anthracnose in greenhouse.[Result]Ten Bacillus strains were selected for their antagonistic effects against E.amylovora.Among them,the three strains with relatively stronger antagonistic activity were PBIB1,PBIB4,and PBIB7.After genome sequencing,PBIB1 and PBIB4 were identified as B.velezensis,while PBIB7 was identified as B.subtilis.These three strains exhibited antagonistic activity against X.oryzae pv.oryzae,R.solanacearum,C.gloeosporioides and F.graminearum,and had the efficacy of 82.83%,78.84%and 68.86%against fire blight,92.31%,90.38%and 67.30%against strawberry anthracnose in greenhouse,respectively.[Conclusion]Three biocontrol Bacillus strains are screened in the mangrove sediments in Fujian Province,which have a broad-spectrum antagonistic effect on crop bacterial and fungal diseases,and have the potential for further development and application of biocontrol agents.关键词
红树林/芽孢杆菌/梨火疫病/拮抗活性/防治效果Key words
Mangrove/Bacillusstrain/Fire blight/Antagonistic activity/Efficacy分类
植物保护学引用本文复制引用
高雅,王美红,杨凤环,刘国红,余超,陈华民..红树林沉积物生防芽孢杆菌资源筛选及防效评价[J].华南农业大学学报,2025,46(2):194-202,9.基金项目
国家自然科学基金(31972255) （31972255）
