江苏农业学报2025，Vol.41Issue(1)：9-20,12.DOI:10.3969/j.issn.1000-4440.2025.01.002
耐盐产吲哚乙酸(IAA)多功能菌株筛选及对盐胁迫小麦的促生效应
Screening of multifunctional salt-tolerant indole-3-acetic acid(IAA)-producing strains and their growth-promoting effects on wheat under salt stress
摘要
Abstract
As a plant hormone,indole-3-acetic acid(IAA)plays an important role in plant seed germination,root development,growth and metabolism.To enrich the resources of IAA-producing strains and investigate their growth-promoting effects under salt stress,multifunctional strains with IAA-producing ability were screened from halophytes in saline-alkali land of Kizilsu Kirghiz Autonomous Prefecture,Xinjiang Uygur Autonomous Region.Then the excellent strains were selected and inoculated into wheat seedlings under salt stress to verify their growth-promoting ability.As a result,a total of 181 IAA-producing strains were isolated,among which MHCA37,MPCB16,and MHCA17 were the top three in terms of IAA secretion.The strains with IAA secretion ability were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),and the representative strains were selected for 16S rRNA gene sequencing.The results showed that all strains be-longed to six genera,and Enterobacter was the dominant genus.The effects of IAA-producing strains on the aboveground and underground parts of wheat under different salt stresses were different.Under 150 mmol/L NaCl stress,the stem diame-ter,plant height,root dry weight,shoot dry weight and chlorophyll content of wheat inoculated with MHCA37,MPCB16 and MHCA17 were significantly higher than those of the control.The catalase(CAT)activity of wheat seedlings inoculated with MHCA37 under 150 mmol/L and 200 mmol/L NaCl treatments was significantly higher than that of the control.The superoxide dismutase(SOD)activity of wheat inoculated with MHCA17 under 150 mmol/L and 200 mmol/L NaCl treat-ments was significantly higher than that of the control.The peroxidase(POD)activity of wheat seedlings inoculated with MHCA37 strain under 150 mmol/L NaCl treatment was significantly higher than that of the control.The POD activity of wheat seedlings inoculated with MHCA17 strain under 200 mmol/L NaCl treatment was significantly higher than that of the control.The malondialdehyde(MDA)content of wheat seedlings inoculated with MHCA37,MPCB16 and MHCA17 strains under 150 mmol/L NaCl mild salt stress was significantly lower than that of the control.It is speculated that the IAA-producing strains screened in this study have great application potential in promoting plant growth and improving plant stress resistance under salt stress.At the same time,the results of this study provide a theoretical basis for the development of salt-tolerant multifunctional microbial agents suitable for saline-alkali land.关键词
吲哚乙酸/多功能菌株/盐胁迫/促生效应Key words
indole-3-acetic acid/multifunctional strains/salt stress/growth-promoting effect分类
农业科技引用本文复制引用
常海霞,李静,李明源,茹仙古丽·尤努斯,王继莲..耐盐产吲哚乙酸(IAA)多功能菌株筛选及对盐胁迫小麦的促生效应[J].江苏农业学报,2025,41(1):9-20,12.基金项目
国家自然科学基金项目(32160408) （32160408）
