江苏农业学报2025，Vol.41Issue(1)：21-27,7.DOI:10.3969/j.issn.1000-4440.2025.01.003
甘蓝型油菜细胞质雄性不育的细胞学观察及不育胞质类型鉴定
Cytological observation and sterile cytoplasm type identification of cyto-plasmic male sterile Brassica napus L.
摘要
Abstract
In order to clarify the pollen abortion mechanism and sterile cytoplasm type of 22N317A,22N195R2 A and 22LJ56A-22N286R1A cytoplasmic male sterile(CMS)lines of winter oilseed rape,three cytoplasmic male sterile lines and maintainer lines of winter oilseed rape were used as materials in this study.The flower morphology and cell characteris-tics were observed,and primers were designed according to the fertility-related genes orf138,orf222 and orf224 of oilseed rape for multiplex PCR molecular identification.The results showed that the petals of the three sterile materials were flat,the anthers could not disperse pollen normally,the filaments were short and the stigmas were long.The development of pol-lens of sterile lines was blocked during the period from tetrad to mononuclear microspore.The results of conventional cyto-logical observation of sterile lines showed that no microspores or pollen grains were observed after the tetrad.Paraffin sec-tions of the sterile line showed that the tapetum expanded abnormally during the pollen tetrad period,squeezing the tetrad in the anther chamber,causing the anther chamber to deform and eventually leading to male sterility.The ster-ile cytoplasm type of the three sterile lines was Ogu NW-SUAF CMS.关键词
甘蓝型油菜/细胞质雄性不育/花器形态/细胞学观察/育性鉴定Key words
Brassica napus L./cytoplasmic male sterility/shape of flower organs/cytological observation/fertili-ty identification分类
生物科学引用本文复制引用
翟利佳,杨馥睿,刘自刚,魏家萍,崔俊美,武泽峰,方彦..甘蓝型油菜细胞质雄性不育的细胞学观察及不育胞质类型鉴定[J].江苏农业学报,2025,41(1):21-27,7.基金项目
国家自然科学基金项目(31860388),甘肃省科技重大专项(22ZD6NA009),甘肃省科技计划项目(23CXJA0001) （31860388）
