江苏农业学报2025，Vol.41Issue(1)：41-50,10.DOI:10.3969/j.issn.1000-4440.2025.01.006
辣椒炭疽病病原菌中分泌蛋白的预测及特性分析
Prediction and characteristic analysis of secretory proteins in pepper an-thracnose pathogens
摘要
Abstract
Pepper anthracnose caused by the pathogens of pepper anthracnose seriously endangers the healthy devel-opment of pepper industry in China.As an important pathogenic factor in anthracnose pathogens,the secretory protein can help pathogens invade host tissues,decompose host cell walls,and provide survival and reproduction con-ditions for pathogens.However,there is still a lack of in-depth analysis and research on the secreted proteins in the pathogens of pepper anthracnose.In this study,based on the whole genome sequence of the strong pathogenic strain TJNH1 of pepper anthracnose pathogens obtained in the previous study,13 419 protein amino acid sequences in the pathogen were predicted by using bioinformatics secreted protein prediction software such as SignalP and ProtComp.Meanwhile,based on transcriptome sequencing data,the functions and characteristics of the above-mentioned secreted pro-teins were studied.The results showed that TJNH1 contained 388 secretory proteins,accounting for 2.89%of the total pro-teins.The length of amino acid sequences of secreted proteins ranged from 55 aa to 730 aa.The non-polar amino acid ala-nine(Ala)was the most frequently used in secreted proteins.The length of the secreted protein signal peptide was concen-trated from 17 aa to 21 aa,and the signal peptide cleavage site was A-X-A type.The secreted proteins were all hydrophilic proteins.Asparagine(Asn)and alanine(Ala)had the largest number of highly hydrophilic amino acids and highly hydro-phobic amino acids,respectively.Most of the secreted proteins were stable and acidic proteins.At the same time,SMART,eggNOG,STRING V11.5 and other software were used to analyze the conserved domains,COG functional classification and protein interaction of the above secreted proteins.There were 152 secreted proteins with obvious conserved domains,163 se-creted proteins were functionally annotated,and 32 secreted proteins had interactions.The results of this study provide a theoretical support for clarifying the functions of secreted proteins and the pathogenic mechanisms of pepper anthracnose pathogens,and also provide a theoretical basis for screening new pesticides targeting secreted proteins and green prevention and control of pepper anthracnose.关键词
辣椒炭疽病病原菌/分泌蛋白/生物信息学分析Key words
pepper anthracnose pathogens/secreted proteins/bioinformatics analysis分类
农业科技引用本文复制引用
李明瑾,霍建飞,韩长志,姚玉荣,贲海燕,郝永娟,王万立..辣椒炭疽病病原菌中分泌蛋白的预测及特性分析[J].江苏农业学报,2025,41(1):41-50,10.基金项目
天津市农业科学院青年科研人员创新研究与实验项目(2022011) （2022011）
云南省"兴滇英才支持计划"青年人才专项(YNWR-QNBJ-2020-188) （YNWR-QNBJ-2020-188）
天津市科技计划项目(23ZYCGSN00220) （23ZYCGSN00220）
