口腔颌面外科杂志2025，Vol.35Issue(1)：28-33,6.DOI:10.12439/kqhm.1005-4979.2025.01.005
紫杉醇联合ipatasertib对口腔鳞状细胞癌细胞死亡、增殖及自噬的影响
The combination of paclitaxel and ipatasertib affects the death,proliferation,and autophagy response of oral squamous cell carcinoma cells
车迪 1徐鹏锟 1宋振宇 1任宝瑞 1关键1
作者信息
- 1. 佳木斯大学附属口腔医院颌面外科,佳木斯 154002
- 折叠
摘要
Abstract
Objective:To investigate the effect of paclitaxel combined with ipatasrtib on the oral squamous cell carcinoma(OSCC)cell line Cal-27 to study the changes of the proliferation level and autophagy level,which will provide the basis for further research and clinical application of oral squamous cell carcinoma(OSCC).Methods:The optical density values of paclitaxel and ipatasertib alone and in combination in Cal-27 cell line were determined by CCK8 assay,and the lethality of the corresponding drugs was further compared to find the most suitable concentration of combination therapy.The expression of autophagy-related protein LC3 among different groups was compared by cell immunofluorescence assay;the autophagy-related protein(mTOR,LC3,Beclin-1,P62)of different groups was observed by Western blotting.Results:Both paclitaxel and ipatasertib alone and in combination promoted the death and proliferation of Cal-27 cells,and the combination resulted in higher cell lethality and inhibition than alone;both paclitaxel and ipatasertib alone and in combination enhanced autophagy in Cal-27 cells,and the combination was more effective than alone.Conclusion:Paclitaxel combined with ipatasertib can promote the death of OSCC cell line Cal-27 and inhibit its proliferation,and the effect was enhanced compared with that of a single drug,and both methods promoted the occurrence of autophagy to varying degrees.关键词
口腔鳞状细胞癌/紫杉醇/ipatasertib/Cal-27细胞/自噬Key words
oral squamous cell carcinoma/paclitaxel/ipatasertib/Cal-27 cells/autophagy分类
口腔医学引用本文复制引用
车迪,徐鹏锟,宋振宇,任宝瑞,关键..紫杉醇联合ipatasertib对口腔鳞状细胞癌细胞死亡、增殖及自噬的影响[J].口腔颌面外科杂志,2025,35(1):28-33,6.
