矿产综合利用2025，Vol.46Issue(1)：86-94,9.DOI:10.3969/j.issn.1000-6532.2025.01.009
耐镉硫酸盐还原菌对镉污染土壤的修复
Remediation of Cadmium-contaminated Soil by Cadmium-resistant Sulfate-reducing Bacteria
摘要
Abstract
In order to investigate the cadmium reduction effect of cadmium-tolerant sulfate-reducing bacteria(SRB)on cadmium-contaminated soil,cadmium-tolerant SRB was screened and isolated using cadmium-tolerant sulfate-reducing sludge as seed sludge,and was injected into soils with different degrees of cadmium contamination(mild,moderate,and severe)to investigate the fugitive morphology of soil cadmium,the changes in sulfate reduction activity and microbial community structure.The results showed that after 53 d of Cd-tolerant SRB remediation,the total Cd content of soils with different Cd contamination levels did not change significantly,the proportion of unstable Cd(exchangeable state,carbonate-bound state)decreased from the initial 45%～68%to 26%～40%,and the proportion of stable Cd(Fe-Mn oxidation state,organic-bound state,residue state)increased significantly from the initial 30%～50%to 60%～75%.The effective passivation of cadmium in soils with different degrees of heavy metal cadmium pollution was achieved.The sulfate content of soils with different degrees of cadmium pollution showed a trend of gradually decreasing and then increasing during the remediation process,while the sulfate reduction activity gradually decreased,and there was regeneration of sulfate in the system.The change trend of each contaminated soil bacterial phylum during the remediation of cadmium-tolerant SRB was more or less the same,with Proteobacteria and Bacteroidetes as the main phylum,Desulfosporosinus and Desulfobacca as the main SRB genera,and their abundance gradually decreased during the remediation process,while sulfur-oxidizing bacteria had a tendency to increase.This shows that cadmium-tolerant SRB can reduce the content of unstable cadmium in cadmium-contaminated soil,and reduce the mobility of cadmium,but it is necessary to optimize the remediation parameters to enhance sulfate reduction and inhibit sulfur oxidation in the remediation process.关键词
重金属镉/硫酸盐还原菌/土壤修复/镉形态分布/群落结构Key words
Heavy metal cadmium/Sulfate reducing bacteria/Soil remediation/Cadmium speciation distribution/Community structure分类
环境科学引用本文复制引用
王鹤,吴明林,秦永丽,郑婉盈,蒋永荣,李学军..耐镉硫酸盐还原菌对镉污染土壤的修复[J].矿产综合利用,2025,46(1):86-94,9.基金项目
国家自然科学基金(52060004) （52060004）
广西高校中青年教师科研基础能力提升项目(2021KY0204) （2021KY0204）
广西区大学生创新创业训练计划项目(201810595169、201810595170、202010595164、202110595063) （201810595169、201810595170、202010595164、202110595063）
