林业科学研究2025，Vol.38Issue(1)：127-138,12.DOI:10.12403/j.1001-1498.20240108
阔叶红松林土壤微生物量及优势树种根际效应对增氮和减水的响应
Soil Microbial Biomass and Rhizosphere Effects of Dominant Tree Species in a Broadleaf Korean Pine Forest in Response to Nitrogen Addition and Water Reduction
摘要
Abstract
[Objective]Increased nitrogen deposition and reduced precipitation due to climate change signi-ficantly affect the structure and function of forest ecosystems.The rhizosphere as the core area of interac-tion among plants,soil,and microorganisms plays a pivotal role in ecosystem processes.however,stud-ies on the effects of nitrogen addition and water reduction on rhizosphere microorganisms remain limited.This study aims to explore the soil microbial biomass and the rhizosphere effects of dominant tree species in response to these changes,in order to predict the adaptation strategies and nutrient cycling mechan-isms of broadleaf Korean pine forests under climate change.[Methods]Two treatments were established for field simulations of nitrogen addition and water reduction:nitrogen addition(50 kg·hm-2·a-1),water re-duction(30%rainfall penetration),and their combination.The"shaking root method"was used to collect rhizosphere and bulk soil samples from Pinus koraiensis and Tilia amurensis.[Result](1)The treatments of nitrogen addition and nitrogen-water combination significantly reduced soil pH while increasing the con-tents of ammonium,nitrate,and total nitrogen in the soil.(2)Nitrogen addition reduced microbial biomass carbon and nitrogen in both the rhizosphere and bulk soil of Tilia amurensis,and microbial biomass phos-phorus in bulk soil.It also decreased microbial biomass nitrogen and phosphorus in the rhizosphere soil of Pinus koraiensis.In contrast,the treatments of water reduction and the combined nitrogen and water re-duction significantly increased microbial biomass phosphorus content in the rhizosphere soil of both spe-cies.(3)The nutrient content and microbial biomass in the rhizosphere soil of both species were higher than those in the bulk soil,demonstrating a positive rhizosphere effect.Moreover,under the treatments of nitrogen addition and nitrogen-water combination,the rhizosphere effect on total carbon,nitrogen,and mi-crobial biomass was greater in Pinus koraiensis than in Tilia amurensis.(4)Rhizosphere soil microbial bio-mass was primarily influenced by nitrate nitrogen and soil moisture content,while microbial biomass in bulk soil was driven by ammonium,nitrate,and total nitrogen.[Conclusion]This study elucidates the response mechanisms of rhizosphere and bulk soil microbial biomass to nitrogen addition and precipitation reduc-tion,as well as the differences in rhizosphere effects among different tree species under environmental changes,providing scientific evidence for future forest management and conservation.关键词
阔叶红松林/土壤微生物量/根际/氮添加/降水减少Key words
broadleaf Korean pine forest/soil microbial biomass/rhizosphere/nitrogen addition/precipitation reduction分类
农业科技引用本文复制引用
刘东玲,王晓春,闫国永,邢亚娟,王庆贵..阔叶红松林土壤微生物量及优势树种根际效应对增氮和减水的响应[J].林业科学研究,2025,38(1):127-138,12.基金项目
国家自然科学基金项目(42230703、42377477) （42230703、42377477）
