林业科学研究2025，Vol.38Issue(1)：160-168,9.DOI:10.12403/j.1001-1498.20240166
香榧WOX基因家族的鉴定与表达分析
Identification and Expression Analysis of WOX Gene Family in Torreya grandis
摘要
Abstract
[Objective]The WOX(Wuschel-related homeobox)gene family is a unique class of plant-specif-ic transcription factors that serve as a stem cell marker genes and play crucial roles in somatic embryogen-esis.Torreya grandis,a conifer species known for producing economically valuable nuts,faces challenges in breeding due to its prolonged growth cycle.This study aims to identify the members of the WOX gene family in Torreya grandis and analyze their expression patterns to identify key WOX genes involved in so-matic embryogenesis.The findings are expected to contribute to enhance understanding of the WOX genes'roles,improve the efficiency of somatic embryogenesis,and support the breeding.[Method]Based on the genome data of Torreya grandis,the WOX genes were identified,and heir physicochemical proper-ties,conserved motifs,conserved domains,and chromosome localization were analyzed.Additionally,we examined the expression patterns of these WOX genes across various tissues,including roots,stems,leaves,callus,and somatic embryos.[Result]A total of 17 WOX family members were identified,which were classified into three phylogenetic clades:the WUS clade,the intermediate clade,and the ancient clade.Each clade contained genes with unique conserved domains.These genes were distributed across six chromosomes.Notably,WOX2,WOX8,WOX9 and WOX13 showed variable expression in roots,stems,leaves,callus and somatic embryos,with notably higher expression levels in somatic embryos and callus.[Conclusion]The relatively higher expression of WOX2,WOX8,WOX9 and WOX13 in somatic em-bryos and calluses suggests that these genes play significant roles in the initiation and maintenance of em-bryogenic callus and the development of somatic embryos in Torreya grandis.关键词
香榧/WOX基因/体细胞胚发生/生信分析/基因表达Key words
Torreya grandis/WOX genes/somatic embryogenesis/bioinformatics analysis/gene expression分类
农业科技引用本文复制引用
王誉,姜筱雯,周小红,吴家胜..香榧WOX基因家族的鉴定与表达分析[J].林业科学研究,2025,38(1):160-168,9.基金项目
光合同化物积累与分配调控香榧产量和品质的机理(U20A2049) （U20A2049）
