北方农业学报2024，Vol.52Issue(6)：13-21,9.DOI:10.12190/j.issn.2096-1197.2024.06.02
平安系列小麦品种(系)穗发芽抗性鉴定及基因的分子标记检测
Identification of pre-harvest sprouting resistance and molecular marker detection of genes in Ping'an series wheat varieties(lines)
摘要
Abstract
[Objective]To investigate the pre-harvest sprouting resistance and genetic composition of Ping'an series wheat varieties(lines).[Methods]The pre-harvest sprouting characteristics of 14 Ping'an series varieties(lines)were analyzed by whole ear germination method,and the sources of pre-harvest sprouting resistance genes were detected by molecular markers of 5 anti pre-harvest sprouting genes.[Results]Among the 14 varieties(lines),the relative pre-harvest sprouting index of Ping'an No.11 was 0.19,reached the level of resistance;the relative pre-harvest sprouting indexes of Ping'an 658 and Ping'an 701 were 0.35 and 0.37,respectively,reached the level of moderately resistance.The results of molecular marker detection of anti pre-harvest sprouting genes showed that the relative pre-harvest sprouting index of haplotype composed of three loci(-222、+646、+666)of TaPHS1 gene was CGAC＜TGAC＜TGAT＜TATT from low to high,and Ping'an No.11 contained the best haplotype of TaPHS1 gene of anti pre-harvest sprouting,7 wheat varieties(lines)including Ping'an 901 contained anti pre-harvest sprouting gene TaVp1B3,4 wheat varieties including Ping'an 12 contain anti pre-harvest sprouting gene PM19A-1,all 14 wheat varieties(lines)contain neither of anti pre-harvest sprouting gene Dorm-1 or Tamyb10-D.[Conclusion]Ping'an No.11 is the only variety with hap-CGAC resistant to pre-harvest sprouting,which can be used in cultivating new varieties of anti pre-harvest sprouting wheat and discovering new anti pre-harvest sprouting gene.关键词
小麦/穗发芽抗性/抗性鉴定/发芽指数/分子标记/等位变异Key words
Wheat/Pre-harvest sprouting resistance/Evaluation of resistance/Germination index/Molecular marker/Allelic variation分类
农业科技引用本文复制引用
吕庆丰,朱昌涛,王刚,吕元丰,闫泽东,张珍,张立琳..平安系列小麦品种(系)穗发芽抗性鉴定及基因的分子标记检测[J].北方农业学报,2024,52(6):13-21,9.基金项目
2023年中原学者工作站(234400510009) （234400510009）
