临床口腔医学杂志2025，Vol.41Issue(2)：67-71,5.DOI:10.3969/j.issn.1003-1634.2025.02.002
Zeb1在小鼠实验性根尖周炎中的表达及作用研究
Expression of Zeb1 in mice apical periodontitis and its possible mechanism
摘要
Abstract
Objective:To investigate the expression and effect of zinc finger E-box-binding protein 1(Zeb1)in mice apical periodontitis.Methods:Apical periodontitis model was induced by exposing the pulp of the mandibular first molars in mice and the mandibles were collected on 0,14,28 days after pulp exposure.H&E and TRAP staining were performed to ob-serve inflammatory infiltration and osteoclast formation in periapical lesions,while micro-CT and high-resolution X-ray ima-ging were used to assess the extent of periapical bone destruction.Zeb1 expression in periapical inflammation and its co-local-ization with macrophages were detected by immunofluorescence double staining.Bone marrow-derived macrophages from wild-type and Zeb1 conditional knockout mice were isolated in vitro to detect their migration and phagocytic functions.Results:A significant increase in inflammatory cells infiltration,osteoclasts formation in periapical lesion,and periapical bone resorption was observed over time following pulp exposure.As the lesion expanded,Zeb1 expression increased rapidly,showing a strong co-localization between the distribution of Zeb1-positive cells and macrophages.Additionally,in vitro experiments revealed a substantial reduction in the phagocytic capacity of Zeb1 conditional knockout macrophages with LPS stimulation(P＜0.05),while their migration ability remained unaffected.Conclusion:Zeb1 expression was significantly and positively correlated with the severity of periapical inflammation,suggesting its involvement in the progression of periapical inflammation through the regulation of macrophage phagocytosis.关键词
锌指E盒结合蛋白1(Zeb1)/巨噬细胞/吞噬功能/根尖周炎Key words
Zinc finger E-box-binding protein 1(Zeb1)/Macrophages/Phagocytosis/Apical periodontitis分类
口腔医学引用本文复制引用
马子涵,林莹,王紫君,孙筱月,朱玲新..Zeb1在小鼠实验性根尖周炎中的表达及作用研究[J].临床口腔医学杂志,2025,41(2):67-71,5.基金项目
国家自然科学基金项目(编号:82370914、81970919、82201042) （编号:82370914、81970919、82201042）
中央高校基本科研业务费专项资金(编号:2042024YXA010) （编号:2042024YXA010）
