北方农业学报2024，Vol.52Issue(6)：39-46,8.DOI:10.12190/j.issn.2096-1197.2024.06.05
黄瓜枯萎病拮抗芽孢杆菌PSYC4的筛选及鉴定
Screening and identification of antagonistic Bacillus PSYC4 from cucumber Fusarium wilt
摘要
Abstract
[Objective]To obtain effective antagonistic biocontrol bacteria against cucumber Fusarium wilt.[Methods]The strains with stronger antagonistic effect to cucumber Fusarium wilt were screened from the samples of Yellow Sea by Oxford Cup method,the effect on germination and seedling of cucumber seeds was determined in greenhouse,the strain with the least influence by Gram staining observe,physiological and biochemical identification and 16S rDNA sequence analysis.[Results]A total of 6 strains with strong antagonistic effect against cucumber Fusarium wilt were screened,including PSYC4,PSYC22,PSYC24,PSYC161,PSYC162 and PSYC132.Among them,strain PSYC4 had the least effect on cucumber seed germination and seedling,with a germination rate of 91.67%and a seedling emergence rate of 100%.The strain PSYC4 was identified as Bacillus pumilus,which had a stronger antagonistic effect on Fusarium oxysporum f.sp.cucumerinum,with an inhibition rate of 69.11%.[Conclusion]Strain PSYC4 can effectively inhibit the Fusarium oxysporum f.sp.cucumerinum,and has a significant biocontrol effect on cucumber Fusarium wilt.关键词
黄瓜枯萎病/海洋细菌/短小芽孢杆菌/黄瓜专化型尖孢镰刀菌Key words
Cucumber Fusarium wilt/Marine bacteria/Bacillus pumilus/Fusarium oxysporum f.sp.cucumerinum分类
农业科技引用本文复制引用
苏洁文,彭晨,管利,孙星..黄瓜枯萎病拮抗芽孢杆菌PSYC4的筛选及鉴定[J].北方农业学报,2024,52(6):39-46,8.基金项目
安徽省科技厅自然科学基金面上项目(2108085MC87) （2108085MC87）
