农业生物技术学报2025，Vol.33Issue(3)：473-485,13.DOI:10.3969/j.issn.1674-7968.2025.03.001
ZmCYP90D1调控玉米叶片褶皱发育的分子机制
Molecular Mechanism of ZmCYP90D1 Regulating Leaf Development in Maize(Zea mays)
摘要
Abstract
The concept of'optimum plant types'in the Green Revolution provides a new idea for the design and breeding of maize(Zea mays)plant type.Over-long and over-wide leaves will lead to decreased yield due to draping,while broad leaves can keep the leaves upright without draping,increase the light receiving area of the population,and effectively improve the photosynthetic efficiency.The classic maize(Zea mays)mutant Crinkly Leaves 1(cr1)showed crinkled leaves and dwarf plants.The following results were obtained after cytological analysis,mapping cloning,gene editing and expression analysis of the mutant.The pavement cells of the mutant's leaves were deformed and irregularly arranged,and the mapping cloning results showed that the target gene ZmCYP90D1 was located within 0.9 Mb at the end of the short arm in chromosome 3.After functional analysis and exon sequencing of 29 coding genes in the region,it was found that the third exon of ZmCYP90D1 was missing in cr1 mutant.Further genome sequencing revealed that a 1.2 kb transposon was inserted at the splicing site at the end of the third exon of the cr1,resulting in the missplicing of the mRNA of ZmCYP90D1 and the loss of the third exon.The ZmCYP90D1(Zm00001d039453)gene encoded cytochrome monooxase,which was involved in the biosynthesis of brassinolactone and located in the endoplasmic reticulum.Gene editing produced 5 homozygous knockout lines,all of which showed crinkly leaf and short plant phenotype.Two of the knockout lines,ko1 and ko2,were analyzed,and ZmCYP90D1 was confirmed as the target gene of cr1.RNA-seq showed differentially expressed genes were mainly concentrated in biological processes such as cell division and differentiation,cell wall formation and modification,hormone regulation response,fatty acid anabolism and so on.The identification of cr1 laid a foundation for further analysis of the molecular mechanism of brassinolide synthesis pathway regulating maize leaf development.关键词
玉米叶片/基因定位/平铺细胞/油菜素内酯Key words
Maize leaves/Gene mapping/Pavement cell/Brassinolide分类
农业科技引用本文复制引用
张诗晗,沈海军,高利,赵婉婷,武小霞..ZmCYP90D1调控玉米叶片褶皱发育的分子机制[J].农业生物技术学报,2025,33(3):473-485,13.基金项目
国家自然科学基金区域创新发展联合基金(U23A20192) （U23A20192）
黑龙江省自然科学基金-研究团队(TD2002C003) （TD2002C003）
