农业生物技术学报2025，Vol.33Issue(3)：513-522,10.DOI:10.3969/j.issn.1674-7968.2025.03.004
机械损伤对马铃薯块茎伤口处的茉莉酸合成和传导的影响
Effect of Mechanical Wounding on Jasmonic Acid Synthesis and Signaling at Potato(Solanum tuberosum)Tuber Wounds
摘要
Abstract
Fruit and vegetables are highly susceptible to mechanical wounding during harvest and postharvest handling,and the wounds induce the production of several phytohormones at the wounds,but involvement of jasmonic acid(JA)in potato(Solanium tuberosum)wound stress has not been reported.In this study,based on transcriptome analysis of pre-wounded potato tubers,the genes related to JA synthesis and signaling were screened,the expression of these genes and related enzyme activities were analyzed,the JA content at the wounds was determined.The results showed that the wound significantly upregulated the expression levels of genes related to JA synthesis at the tuber wound,including lipoxygenase(LOX)family members StLOX1.5,StLOX2,StLOX3.1 and StLOX6,propadiene oxidative synthase(AOS)family members StAOS1,StAOS2 and StAOS3,and propadiene cyclohexylase(AOC)family members StAOC and 12-oxo-dienoic acid reductase(OPR)family members StOPR1,StOPR2 and StOPR3.The wound also significantly increased LOX and AOS activities and significantly increased JA levels at the tuber wounds.In addition,the wound significantly upregulated the expression levels of key JA signaling genes,the jasmonate ZIM domain(JAZ)family members StJAZ1,StJAZ2,StJAZ3,StJAZ4 and StJAZ7.In conclusion,the wound activates JA synthesis and signaling in potato tuber wounds.This study provides a theoretical basis for the study of the response mechanism of potato wound stress.关键词
马铃薯块茎/机械损伤/茉莉酸(JA)/合成/传导Key words
Potato tubers/Mechanical wounding/Jasmonic acid(JA)/Synthesis/Signaling分类
农业科学引用本文复制引用
白露,杨瑞瑞,李芳红,王颖,王雪雪,张雪姣,韩叶,毕阳..机械损伤对马铃薯块茎伤口处的茉莉酸合成和传导的影响[J].农业生物技术学报,2025,33(3):513-522,10.基金项目
国家自然科学基金(32360784) （32360784）
