农业生物技术学报2025，Vol.33Issue(3)：536-546,11.DOI:10.3969/j.issn.1674-7968.2025.03.006
PpMYB113基因在桃花青苷合成和积累中的功能分析
Functional Analysis of the PpMYB113 Gene in Anthocyanin Synthesis and Accumulation in Peach(Prunus persica)
摘要
Abstract
MYB transcription factors play a crucial role in the accumulation of anthocyanins in peach(Prunus persica)fruit.A deeper understanding of MYB transcription factors is beneficial for elucidating the molecular regulatory mechanism of secondary metabolic networks in peach,providing a theoretical basis for genetic improvement,and developing new varieties rich in anthocyanins.In this study,in order to investigate the role of PpMYB113 in the accumulation of anthocyanins in peach flowers and fruits,the PpMYB113 gene(GenBank No.XM_007216468.2)was cloned using red flesh peach as the experimental material.The bioinformatics and expression pattern analysis was performed,and the function of PpMYB113 gene was studied through transient transformation in tobacco(Nicotiana benthamiana)and peach flesh.The results showed that the open reading frame of PpMYB113 gene was 720 bp,encoding 239 amino acids.The phylogenetic tree analysis indicated that the PpMYB113 protein sequence had the closest phylogenetic relationship with the MYB transcription factors of the S6 subfamily in Arabidopsis thaliana.The expression of PpMYB113 gene was analyzed in petals,branches,leaves,bark,fruit skin,and fruit flesh of peach by using semi-quantitative RT-PCR and RT-qPCR.The results indicated that PpMYB113 gene was highly expressed in petals,fruit skin,and fruit flesh,while it was not expressed in branches,leaves,and bark.At the same time,PpMYB113 was overexpressed in tobacco leaves and white fruit flesh using the transient transformation system.The overexpressed tobacco leaves and white fruit flesh showed red color phenotype and the anthocyanin contants were significant increase.In the overexpressed tobacco leaves and white fruit flesh areas,the expression of PpMYB113 was significantly higher than that of the control,and the expression of ANS(anthocyanidin synthase)and UFGT(UDP glucose:flavonoid-3-O-glucosyltransferase)genes were significantly up-regulated.Based on these findings,it was concluded that PpMYB113 plays a positive promoting role in the accumulation of anthocyanins in peach.These results provide a foundation for further investigation into the molecular regulatory mechanism of MYB transcription factors in the accumulation of anthocyanin in red flesh peach.关键词
桃/MYB转录因子/表达分析/花青苷Key words
Peach/MYB transcription factors/Expression analysis/Anthocyanins分类
农业科技引用本文复制引用
周淑婷,李茂福,王华,孙佩,康岩慧,孙向一,武军凯,张立彬,金万梅..PpMYB113基因在桃花青苷合成和积累中的功能分析[J].农业生物技术学报,2025,33(3):536-546,11.基金项目
北京市农林科学院科技创新平台建设项目(PT2023-07) （PT2023-07）
北京市农林科学院科技创新能力专项(KJCX20230602) （KJCX20230602）
河北省现代农业产业技术体系创新团队建设(HBCT2024160203) （HBCT2024160203）
