农业生物技术学报2025，Vol.33Issue(3)：570-580,11.DOI:10.3969/j.issn.1674-7968.2025.03.009
薰衣草萜类合成相关基因LiMYC1的表达分析及功能验证
Expression Analysis and Functional Verification of the LiMYC1 Related to Lavender(Lavandula × intermedia)Terpenoid Synthesis
摘要
Abstract
Lavender(Lavandula angustifolia)is an important spice cash crop in the world.The essential oil extracted from the inflorescence is rich in aromatic compounds.These compounds mainly rely on the methylerythritol-4-phosphate(MEP)pathway in plant plastids for metabolic synthesis.In the MEP pathway,1-deoxy-D-xylose-5-phosphate synthase(DXS)and 1-deoxy-D-xylulose 5-phosphate reductoisomerase(DXR),as the first 2 key rate-limiting enzymes,play a decisive role in the smooth progress of the whole metabolic process.The LiMYC1(myelocytomatosis 1)gene cloned from Lavandula × intermedia was targeted,and its function in the synthesis of volatile terpenoids was verified.In this study,The pCAMBIA3301 and pTRV2 plant expression vectors were constructed,and overexpression and gene-silencing plants were obtained using Agrobacterium-mediated genetic transformation methods.The expression of LiMYC1,LiDXS and LiDXR genes in transgenic plants were detected,and volatile terpenoids in leaves were qualitatively and quantitatively analyzed.The results showed that in the LiMYC1 gene overexpression line,the expression of LiDXS and LiDXR increased significantly,and the contents of linalool and caryophyllene also increased to varying degrees.On the contrary,in the LiMYC1 gene silenced strain,the expression of LiDXS and LiDXR were significantly reduced,and the content of caryophyllene was reduced,but the content of linalool was abnormally increased.The above results indicated that the LiMYC1 gene positively regulated the MEP pathway of terpenoid synthesis,affected the expression of key enzymes LiDXS and LiDXR,and had a certain regulatory effect on the biosynthesis of caryophyllene and linalool.This study has important guiding significance for analyzing the molecular mechanism of lavender aroma formation and optimizing the economic value of lavender.关键词
薰衣草/萜类化合物/转录因子/功能验证Key words
Lavender/Terpenoids/Transcription factor/Functional verification分类
农业科学引用本文复制引用
薛志航,买尔孜亚古丽·阿不来克木,王爱凡,廖燕,严中建,苏秀娟,郭丹丽..薰衣草萜类合成相关基因LiMYC1的表达分析及功能验证[J].农业生物技术学报,2025,33(3):570-580,11.基金项目
新疆维吾尔自治区"天山英才"青年拔尖人才项目(2023TSYCCX0017) （2023TSYCCX0017）
国家自然科学基金地区科学基金(31960452) （31960452）
