农业生物技术学报2025，Vol.33Issue(3)：581-593,13.DOI:10.3969/j.issn.1674-7968.2025.03.010
木麻黄海藻糖-6-磷酸合酶家族鉴定及表达分析
Identification and Expression Analysis of Trehalose-6-Phosphate Synthase Family in Casuarina equisetifolia
摘要
Abstract
Trehalose-6-phosphate synthase(TPS)is the crucial rate-limiting enzyme for the trehalose biosynthesis,and plays a significant role in the stress responsiveness.To investigate the function of TPS regarding growth and development,stress response in Casuarina equisetifolia,physicochemical features,chromosomal localization,phylogenetic tree,conserved motifs,gene structure,cis-regulatory elements and collinearity were analyzed in this study.Their expression patterns in different tissues,and exposed to drought,salt stress and abscisic acid(ABA)treatment were determined.Results indicated that 7 CeTPS genes were randomly distributed in 4 chromosomes,encoding 826～998 amino acids that were hydrophilic.The CeTPS proteins were divided into 2 subfamilies through phylogenetic analysis,showing relatively close to dicotyledonous plants,and harbored gene duplications.The promoter regions of CeTPS genes were rich in cis-acting elements involved in hormonal responsiveness,growth and development,and stress responsiveness.CeTPS genes showed tissue-specific expression,and were differentially expressed under drought,salt stress,and ABA treatment.Especially,CeTPS4 might play an essential role in stress responsiveness.The results lay a foundation for in-depth study of the CeTPS genes associated with abiotic stress responsiveness.关键词
木麻黄/海藻糖-6-磷酸合酶(TPS)/表达分析/胁迫Key words
Casuarina equisetifolia/Trehalose-6-phosphate synthase(TPS)/Expression analysis/Stress分类
农业科技引用本文复制引用
张桂华,岳春雷,李贺鹏,张晓勉,俞振明..木麻黄海藻糖-6-磷酸合酶家族鉴定及表达分析[J].农业生物技术学报,2025,33(3):581-593,13.基金项目
浙江省省院合作林业科技项目(2022SY06) （2022SY06）
国家自然科学基金(32401543) （32401543）
浙江省省属科研院所扶持专项(2024F1068-2) （2024F1068-2）
浙江省基础公益研究计划项目(LY23H280003) （LY23H280003）
