农业生物技术学报2025，Vol.33Issue(3)：608-615,8.DOI:10.3969/j.issn.1674-7968.2025.03.012
湖羊TMEM18基因的组织表达及其多态性与饲料转化率的关联分析
Tissue Expression of TMEM18 Gene and Correlation Analysis Between Its Polymorphism and Feed Conversion Ratio in Hu Sheep(Ovis aries)
摘要
Abstract
Transmembrane protein 18(TMEM18)is an important candidate gene for animal growth and development,encoding a transmembrane protein with important biological functions.In order to explore the expression pattern of TMEM18 gene in sheep(Ovis aries)tissues,the correlation between it's polymorphism and feed conversion ratio(FCR),and molecular genetic markers for breeding were searched.Based on a population of 845 Hu sheep with clear pedigrees and complete phenotypic records,this research utilized qRT-PCR technology to detect the expression levels of the TMEM18 gene in 10 tissues of Hu sheep and employed Sanger sequencing technology to scan for SNPs in this gene,which were then analyzed for association with feed efficiency traits.The results showed that the relative expression of the TMEM18 gene was significantly higher in the heart than in other tissues(P＜0.05),followed by high relative expression in the duodenum,rumen,lungs,and liver.A synonymous mutation polymorphism site,g.517832 T＞C,was detected in the third intron of the sheep TMEM18 gene.The results of multiple comparisons showed that the mutation locus was significantly associated with FCR100～120,FCR140～160,FCR80～120,FCR140～180,FCR100～160,FCR80～160,FCR100～180 and FCR80～180(P＜0.05),among them,FCR140～160,FCR100～160,FCR80～160,FCR100～180 and FCR80～180 with CC genotype were significantly higher than those of TT genotype and TC genotype(P＜0.05).The results of this study suggested that TMEM18 g.517832 T＞C site could be used as a candidate molecular marker to improve feed efficiency in sheep.This study provides reliable genetic materials for the breeding of grain-saving varieties.关键词
跨膜蛋白18(TMEM18)基因/饲料转化率/湖羊/SNPsKey words
Transmembrane protein 18(TMEM18)gene/Feed conversion ratio/Hu sheep/SNPs分类
畜牧业引用本文复制引用
刘晓强,蔡有鑫,张小雪,李成海,王维民,高飞,田慧彬,王立忠,赵源,马宗武,黄志强..湖羊TMEM18基因的组织表达及其多态性与饲料转化率的关联分析[J].农业生物技术学报,2025,33(3):608-615,8.基金项目
国家重点研发计划(2022YFD1302000) （2022YFD1302000）
甘肃省科技重大专项(22ZD6NC069) （22ZD6NC069）
金川集团股份有限公司2023年重大科研项目(金科综2023-13) （金科综2023-13）
节粮低脂湖羊的选育和研究(JKQGJJ23Z202301) （JKQGJJ23Z202301）
