农业生物技术学报2025，Vol.33Issue(3)：642-649,8.DOI:10.3969/j.issn.1674-7968.2025.03.015
鸭沙门菌rfaH基因缺失株的构建及其生物学特性研究
Construction of the rfaH Gene Deletion Strain in Salmonella anatum and Study of Its Biological Characteristics
摘要
Abstract
Salmonella anatum is a major pathogen that causes salmonellosis in ducks(Anatidae).rfaH encodes the transcriptional anti-termination factor RfaH,which is essential for bacterial transcription,infection,and other biological functions.This study investigated the effect of the rfaH gene on various biological aspects of S.anatum,including growth,motility,biofilm formation,and cell infection.To deeply understand the biological roles of the rfaH gene in S.anatum,an rfaH gene deletion strain △rfaH and the complement strain C△rfaH derived from the S.anatum isolate strain SA01 were generated using Red homologous recombination technology.The sequencing results showed that the rfaH gene deletion strain△rfa H and the complement strain C△rfaH of SA01 were successfully constructed.After the deletion of the rfbH gene,the growth rate remained unchanged,yet the O-antigen synthesis was impaired,leading to a significant decrease in bacterial motility,an enhancement in biofilm formation,an increased adhesion and invasion capacity against DF-1 cells,and a weakened proliferation ability in RAW264.7 cells.The findings indicated that the rfaH gene was involved in bacterial motility,biofilm formation,and cell infection.This study establishes a solid platform for future research on the biological functions of the rfaH gene and the development of Salmonella-based delivery vectors for vaccine applications.关键词
鸭沙门菌/rfaH基因/生物学特性Key words
Salmonella anatum/rfaH/Biological characteristics分类
农业科技引用本文复制引用
宋亚雯,韩月,高宇杰,张晓荟,王温馨,葛同鑫,翟瑞东,宋厚辉,程昌勇,李爱云..鸭沙门菌rfaH基因缺失株的构建及其生物学特性研究[J].农业生物技术学报,2025,33(3):642-649,8.基金项目
国家重点研发计划项目(2023YFD1801000) （2023YFD1801000）
浙江省自然科学基金(LQ24C010005) （LQ24C010005）
国家自然科学基金(32102671131902280) （32102671131902280）
