口腔颌面外科杂志2025，Vol.35Issue(1)：7-13,7.DOI:10.12439/kqhm.1005-4979.2025.01.002
Lmo7对ATDC5细胞体外成软骨分化及增殖、迁移影响的实验研究
Effects of Lmo7 on chondrogenic differentiation,proliferation and migration of ATDC5 cells in vitro
摘要
Abstract
Objective:To investigate the effects of LIM domain 7(Lmo7)on chondrogenic differentiation,proliferation and migration of ATDC5 cells in vitro.Methods:Incubate ATDC5 cells in vitro,cell immunofluorescence was used to determine the location of Lmo7 in ATDC5 cells.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the genetic expression of Lmo7 during chondrogenic differentiation.ATDC5 cells was transfected with small interfering RNA(siRNA)to inhibit the genetic expression level of Lmo7,markers of chondrogenic differentiation were detected by RT-qPCR after chondrogenic induction.The effect of Lmo7 knockdown on proliferation was observed by CCK8 assay.Wound-healing assay was used to determine the effect of Lmo7 knockdown on migration.Results:Lmo7 is located in the nucleus and cytoplasm of ATDC5 cells.After chondrogenic induction in vitro,Lmo7 upregulated significantly together with chondrogenic marker.After knockdown of Lmo7 by siRNA,chondrogenic marker SRY-related high mobility group-box gene 9(Sox9),typeⅡ collagen(Col2)upregulated significantly.CCK8 assay showed that the proliferation of knockdown group was higher than that of control group.In wound-healing assay,the healing efficiency of knockdown group was lower than that of control group.Conclusion:Lmo7 is upregulated during chondrogenic differentiation in ATDC5 cells.Knockdown of Lmo7 leads to promotion of chondrogenic differentiation of ATDC5 cells.Inhibiting the expression level of Lmo7 promoted proliferation but inhibited migration of ATDC5 cells.关键词
Lmo7/ATDC5细胞/分化/增殖/迁移Key words
Lmo7/ATDC5 cells/differentiation/proliferation/migration分类
医药卫生引用本文复制引用
陶然,王佐林..Lmo7对ATDC5细胞体外成软骨分化及增殖、迁移影响的实验研究[J].口腔颌面外科杂志,2025,35(1):7-13,7.基金项目
科技部重点研发专项(2018YFE0202200) （2018YFE0202200）
国家自然科学基金(81600836) （81600836）
