口腔颌面外科杂志2025，Vol.35Issue(1)：14-20,7.DOI:10.12439/kqhm.1005-4979.2025.01.003
牵张力对大鼠髁突纤维软骨干细胞成软骨分化的影响
Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells
摘要
Abstract
Objective:To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells(FCSCs).Methods:Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs,and flow cytometry was conducted to examine the surface markers of rat condylar FCSCs.Afterward,the rat condylar FCSCs were stretched with a strength of 10% for 48 h,and the expression of related chondrogenic genes was detected by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blotting.We also established rat mandibular advancement(MA)model to find out the in vivo effect of mechanical stretch on the superficial layer of the condylar cartilage.Results:The rat condylar FCSCs could differentiate towards adipogenesis,osteogenesis,and chondrogenesis,and they expressed mesenchymal markers CD90 and CD29,while negative for hemopoietic markers CD45 and CD11.Compared with the Control group,the Stretch group expressed a higher level of SRY-related high mobility group-box 9(Sox9),proteoglycan 4(Prg4),and typeⅠcollagen(Col1)genes,while a lower level of matrix metalloproteinase 13(MMP13)gene.In vivo,mechanical stretch could increase the superficial layer thickness in the rat condylar cartilage compared with the Control group.Conclusion:Mechanical stretch can promote the chondrogenic differentiation of rat condylar FCSCs and the cartilage matrix formation in the superficial layer of the condylar cartilage.关键词
牵张力/髁突/纤维软骨干细胞/成软骨分化Key words
mechanical stretch/condyle/fibrocartilage stem cells/chondrogenic differentiation分类
医药卫生引用本文复制引用
龚吴仪,米晓晖,李永明..牵张力对大鼠髁突纤维软骨干细胞成软骨分化的影响[J].口腔颌面外科杂志,2025,35(1):14-20,7.基金项目
国家自然科学基金(81970921) （81970921）
