口腔颌面外科杂志2025，Vol.35Issue(1)：21-27,7.DOI:10.12439/kqhm.1005-4979.2025.01.004
NUDT1促进口腔鳞状细胞癌细胞增殖的体外实验研究
In vitro experimental study of NUDT1 promoting the proliferation of oral squamous cell carcinoma cells
摘要
Abstract
Objective:To investigate the effect of Nudix hydrolase 1(NUDT1)on the proliferation of oral squamous cell carcinoma(OSCC)cells,and its mechanism of function was tentatively explored.Methods:Immunohistochemistry was applied to examine paraffin sections of 30 patient tissues with OSCC and 10 normal oral mucosa tissues.The expression of NUDT1 in OSCC cells and normal oral mucosal epithelial cells was detected by Western blotting.In addition,the expression of NUDT1 was down-regulated by small interfering RNA(siRNA)interference.The effect of NUDT1 silencing on cell proliferation in OSCC cell lines CAL27 and HN30 was examined by CCK8 assay,and the effect of NUDT1 silencing on cell apoptosis was analyzed by immunofluorescence through an apoptosis kit.Results:Immunohistochemistry assay showed that NUDT1 expression was higher in OSCC tissues than that in normal oral mucosa tissues,and Western blotting assay revealed that NUDT1 expression was elevated in OSCC cells(CAL27 and HN30 cells)compared with that in normal oral mucosal epithelial cells(P<0.01).Inhibition of NUDT1 gene expression significantly inhibited the proliferation ability of OSCC cells(P<0.05),and promoted apoptosis of OSCC cells.Conclusion:NUDT1 expression was elevated in OSCC tissues,compared to that in normal oral mucosa tissues.Downregulation of NUDT1 was important for inhibiting the proliferation of OSCC cells and promoting apoptosis of OSCC cells.关键词
口腔鳞状细胞癌/Nudix水解酶1/增殖/凋亡Key words
oral squamous cell carcinoma/Nudix hydrolase 1/proliferation/apoptosis分类
医药卫生引用本文复制引用
郭浩兵,鲁莉莉,郝雅翠,赵博文,高艺涵,朴松林..NUDT1促进口腔鳞状细胞癌细胞增殖的体外实验研究[J].口腔颌面外科杂志,2025,35(1):21-27,7.基金项目
哈尔滨医科大学附属第一医院科研创新基金(2020M13) （2020M13）
