山西中医药大学学报2025，Vol.26Issue(2)：144-151,8.DOI:10.19763/j.cnki.2096-7403.2025.02.04
基于靶向检测分析大黄素对正常小鼠肝组织中脂肪酸代谢的影响
Effect of emodin on fatty acid metabolism in normal mouse liver tissues based on targeted detection
摘要
Abstract
Objective:To establish a targeted detection method for fatty acid(FFA)content in mouse liver tissues,in-vestigate the methodology,and provide scientific data for the clinical application of emodin.Methods:32 mice were ran-domly divided into blank group(C),low-dose emodin group(C_L),medium-dose emodin group(C_M),high-dose emodin group(C_H),with 8 mice in each group.Emodin was administered at 40 mg/kg,80 mg/kg and 120 mg/kg once a day for 4 weeks.Ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS)was used to detect the changes of FFA content in mouse liver tissues.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed on the original data using SIMCA 14.1 software to compare the difference of FFA con-tent between groups.Differential metabolites were screened according to variable importance projection(VIP)>1.0.The ef-fects of emodin on FFA metabolism in mice were visualized by heat map analysis.Results:There was no statistical signifi-cance in FFA content in mouse liver tissues between C_L group and C group(P>0.05).The contents of Linoleic acid[FFA(18:2)]and arachidonic acid[FFA(20:4)]in mouse liver tissues in C_M group and C_H group were significantly higher than those in C group,and the difference was statistically significant(P<0.01),which indicated that Omega-6 polyunsatu-rated fatty acids(n-6 PUFAs)were accumulated in mouse liver tissues in C_M and C_H groups.Compared with those of C group,FFA(20:4)/docosahexaenoic acid[FFA(22:6)]and palmitic acid[FFA(16:0)]/FFA(22:6)in mouse liver tissue in C_L group,C_M group and C_H group were increased,and the differences were statistically significant(P<0.01).Con-clusion:FFA(18:2),FFA(18:0),FFA(18:1),FFA(16:0),FFA(20:4),FFA(20:4)/FFA(22:6),and FFA(16:0)/FFA(22:6)may be potential biomarkers of emodin regulation of FFA metabolism in liver tissues.关键词
大黄素/脂肪酸/靶向检测/肝组织Key words
emodin/FFA/targeted detection/liver tissues分类
医药卫生引用本文复制引用
毛睿,赵付霞,蔡亚楠,任超云,张紫媛,王颖莉..基于靶向检测分析大黄素对正常小鼠肝组织中脂肪酸代谢的影响[J].山西中医药大学学报,2025,26(2):144-151,8.基金项目
山西省中医药管理局省教育规划办项目(2023ZYYB2035) （2023ZYYB2035）
山西省基础研究计划自由探索青年项目(202303021212238) （202303021212238）
山西中医药大学博士科研启动基金项目(2023BK12) （2023BK12）
山西中医药大学优秀博士毕业生来晋工作奖励经费科研启动基金项目(2023BKS21) （2023BKS21）
