山西中医药大学学报2025，Vol.26Issue(2)：160-169,174,11.DOI:10.19763/j.cnki.2096-7403.2025.02.06
基于UHPLC-Q-TOF-MS非靶向代谢组学的远志木心差异化学成分研究
Study on the differential chemical compositions of the xylem of Yuanzhi using UHPLC-Q-TOF-MS-based non-targeted metabolomics
摘要
Abstract
Objective:To elucidate the differential chemical compositions between the phloem and xylem of Yuanzhi based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS)non-targeted metabolomics technology.Methods:UHPLC-Q-TOF-MS was used to collect data on the xylem and phloem samples of Yuanzhi.The data processing and statistical analysis were carried out successively in SCIEX Chinese herbs database retrieval,GNPS molecular network construction and annotation,metabolomics data processing,and multivariate statistical analysis to analyze the chemical compositions of the xylem and phloem of Yuanzhi.Non-targeted metabolomics technology was used to analyze the different chemical compositions of the phloem and xylem of Yuanzhi.Results:A total of 117 ingredients were identified,including 24 triterpene saponins,10 ketones,24 sugar esters,8 flavones,10 amino acids,29 organic acids(esters),1 lipid,2 nucleosides(acids),1 lignans,1 phenylpropyl,1 alkaloid,3 sugars and 3 other compounds.Among them,69 components were identified by negative ion model,including 22 triterpenoid saponins,7 ketones,3 flavones,9 or-ganic acids(esters),23 sugar esters,3 sugars and 2 other compounds,and 31 different metabolites,such as 3,6 '-sinaloyl sucrose,Tenuifoliside A/B and so on.48 components were identified by positive ion model,including 2 triterpene saponins,3 ketones,1 sugar ester,5 flavones,10 amino acids,20 organic acids(esters),1 lipid,2 nucleosides(acids),1 lignan,1 phenylpropanoid,1 alkaloid and 1 other compound,and 10 different metabolites,such as oleic acid,senegenin B,etc.Con-clusion:This study opens a new perspective for the related research that whether the xylem should be removed from Yuanzhi,and lays a foundation for the quality control research related to the safety of Yuanzhi.关键词
远志木心/服之令人闷/UHPLC-Q-TOF-MS/非靶向代谢组学/差异化学成分Key words
xylem of Yuanzhi/"MEN"effects/UHPLC-Q-TOF-MS/non-targeted metabolomics/differential chemi-cal compositions分类
医药卫生引用本文复制引用
李娟,娄迎昕,张丽君,马佳琦,毛伟,马运治,王海花,郭丁丁,张福生..基于UHPLC-Q-TOF-MS非靶向代谢组学的远志木心差异化学成分研究[J].山西中医药大学学报,2025,26(2):160-169,174,11.基金项目
山西省基础研究计划自然科学研究面上项目(202403021211184) （202403021211184）
山西省2025年度中医药产业发展现代化支撑项目(2025zyycy04) （2025zyycy04）
山西省中医药管理局科研课题(2024ZYY2A030) （2024ZYY2A030）
山西省中医药管理局科研课题(2024ZYYA001) （2024ZYYA001）
山西省科技成果转化引导专项(202104021301064) （202104021301064）
吕梁市引进高层次科技人才重点研发项目(2021RC-2-13) （2021RC-2-13）
