色谱2025，Vol.43Issue(3)：245-251,7.DOI:10.3724/SP.J.1123.2024.06011
离子色谱法测定呼出气和唾液中的葡萄糖
Determination of glucose in exhaled breath and saliva by ion chromatography
摘要
Abstract
A novel noninasive method was developed for determining glucose levels in human exhaled breath and saliva using ion chromatography.This innovative approach involves collec-ting exhaled breath and saliva samples using a self-designed condensation device and non-stimulative method to ensure minimal participant discomfort.The glucose contents in both ex-haled breath condensate(EBC)and saliva were analyzed using ion chromatography,which is highly sensitive and specific.The experimental conditions were optimized,including a conden-sation temperature of-14℃and an expiratory flow of 15 L/min.A Dionex CarboPac MA1 ion chromatography column(250 mm×4 mm)was used to separate glucose,with the column tem-perature maintained at 30℃.Sodium hydroxide solution(0.8 mol/L)with a pump flow rate of 0.4 mL/min was used as the mobile phase for ion chromatography.Under these conditions,glucose exhibited a good linear relationship in the range of 0.01-20 mg/L,with a correlation coefficient of 0.999 9,along with limits of detection(LOD)and quantification(LOQ)of 2.1 and 7.0 μg/L,respectively.The intra-and inter-day precisions of glucose content in exhaled breath and saliva samples of≤7.5% (n=5)and≤8.4% (n=5),respectively.The results re-veal that the glucose levels in exhaled breath and saliva are strongly correlated with blood glu-cose levels.The method was validated by measuring the glucose contents of exhaled breath and saliva from six diabetic patients and six healthy subjects.Little variation in the glucose contents of the exhaled breath of the two groups was observed under fasting states.However,the ex-haled breath of the diabetic patients exhibited significantly higher(by factors of 6-80)glucose contents(48.4-140.0 ng/L)than those of healthy subjects(1.7-7.9 ng/L)1 h after glucose ingestion.Saliva samples from fasting diabetic patients contained 1.2-5.0-times more glucose contents(87.6-158 mg/L)than those of healthy subjects(31.6-70.9 mg/L).In addition,the saliva of the diabetic patients exhibited glucose contents(136-257 mg/L)that were 1.8-7.7-times higher than those of the healthy subjects(33.1-75.2 mg/L)2 h after glucose ingestion.The developed method provides a simple,precise,and non-invasive means of detecting glucose contents in a manner that does not harm the human body;hence,it is a promising non-invasive metabolic-monitoring tool.This study opens new avenues for the development of innovative technologies for monitoring glucose and other biomarkers,which is expected to greatly enhance metabolic-study accuracy and ease,particularly in the context of managing diabetes and other metabolic disorders.关键词
冷凝收集/离子色谱/呼出气/唾液/葡萄糖/糖尿病Key words
condensation collection/ion chromatography(IC)/exhaled breath condensate(EBC)/saliva/glucose/diabetes分类
化学引用本文复制引用
许健军,楼超艳,卓延红,朱岩..离子色谱法测定呼出气和唾液中的葡萄糖[J].色谱,2025,43(3):245-251,7.基金项目
国家自然科学基金项目(22304167) （22304167）
浙江省自然科学基金项目(LTGC23B050005).National Natural Science Foundation of China(No.22304167) （LTGC23B050005）
Zhejiang Provincial Natural Science Founda-tion(No.LTGC23B050005). （No.LTGC23B050005）
