色谱2025，Vol.43Issue(3)：275-282,8.DOI:10.3724/SP.J.1123.2024.07009
开放性实验:基于超高效液相色谱-串联质谱法的甲状腺癌组织切片蛋白质组定量分析
Open experiment:quantitative proteomics analysis of thyroid-cancer tissue slices using ultra-high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
Open experiments play crucial roles in developing undergraduate students'practical abilities,innovative thinking,and teamwork.This open experiment is designed to use ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)in pro-teomics,a frontier field,by quantitatively analyzing thyroid-cancer-tissue-slice samples.Vari-ous reagents are first screened for further study by evaluating their abilities to extract proteins.This approach aims to foster students'hands-on abilities and their scientific-research thinking.The liquid-chromatography method is subsequently optimized to enable deep-coverage of the proteome,thereby enhancing students'understanding of liquid chromatography and mass spectrometry.Paraffin-embedded thyroid-cancer-tissue slices are finally subjected to quantita-tive proteomic analysis,resulting in the identification of 33 differentially expressed proteins,thereby demonstrating their potential use in disease-typing applications.This open experiment integrates theoretical and experimental knowledge gained through instrumental analysis,analyt-ical chemistry,and biochemistry,thereby imparting scientific research thinking and innovative spirit to undergraduates.It also provides students with opportunities to expand and solidify the-oretical knowledge through hands-on instrumental operation and experiments,which helps to build a systematic knowledge framework and develop a comprehensive understanding of rele-vant fields.Moreover,it stimulates undergraduate interest in scientific research,cultivates in-novative thinking,and fosters team cooperation.关键词
液相色谱/质谱/蛋白质组学/无标记定量/实验教学Key words
liquid chromatography(LC)/mass spectrometry(MS)/proteomics/label-free quantification/experimental teaching分类
化学引用本文复制引用
李一岚,袁辉明,曹竞天,郑怡迪,李兰,高培峰..开放性实验:基于超高效液相色谱-串联质谱法的甲状腺癌组织切片蛋白质组定量分析[J].色谱,2025,43(3):275-282,8.基金项目
北京理工大学实验室研究项目(2023BITSYA04,2021BITSYC11) （2023BITSYA04,2021BITSYC11）
中国科学院分离分析化学重点实验室开放基金(KL2301).Laboratory Research Project of Beijing Institute of Technology(Nos.2023BITSYA04,2021BITSYC11) （KL2301）
Open Fund Project of Key Laboratory of Separation and Analytical Chemistry,Chinese Academy of Sciences(No.KL2301). （No.KL2301）
