生物技术通报2025，Vol.41Issue(2)：30-39,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0526
DNA甲基转移酶在表观遗传学中的应用研究进展
Research Progress in the Application of DNA Methyltransferases in Epigenetics
摘要
Abstract
DNA methyltransferases are a class of enzymes that play a key role in the process of DNA methylation.In living organisms,DNA methyltransferases add methyl groups to nucleic acid molecules,introduce DNA methylation modifications,alter epigenetic modifications,and regulate gene expression.DNA methyltransferases can be in fusion recombination expression in vitro and introduce into cells to have artificial DNA methylation modifications on chromatin.Taking advantage of this feature,in recent years,researchers have developed a variety of epigenetic sequencing technologies based on DNA methyltransferases by methylation labeling of specific locations in the genome,combined with high-throughput sequencing.These applications include chromatin accessibility measurement,nucleosome localization,transcription factor footprints,and histone modification assays.These techniques can obtain not only epigenetic information,but also genomic information and endogenous DNA methylation information,thus they have gradually become the important methods of epigenetics.In this paper,we briefly introduce the previous research methods,review the principles and applications of a variety of recent technologies based on DNA methyltransferase for the detection of epigenetic modifications,and discuss and prospect their future development,aiming to provide a reference for epigenetic research.关键词
DNA甲基转移酶/DNA甲基化/染色质可及性/DNA-蛋白质互作/测序技术Key words
DNA methyltransferase/DNA methylation/chromatin accessibility/DNA-protein interaction/sequencing technology引用本文复制引用
周文健,崔晓楠,施威扬..DNA甲基转移酶在表观遗传学中的应用研究进展[J].生物技术通报,2025,41(2):30-39,10.基金项目
国家自然科学基金项目(41676119,32370863) （41676119,32370863）
