色谱2025，Vol.43Issue(3)：289-294,6.DOI:10.3724/SP.J.1123.2024.04025
仪器分析综合实验:电感耦合等离子体质谱法测定镓元素
Comprehensive instrumental analysis experiment:determination of gallium using inductively coupled plasma mass spectrometry
摘要
Abstract
"Admiring the lofty mountains,one realizes their own lack of talent."To further promote the implementation of new curriculum standards,a comprehensive instrumental analy-sis experiment was designed.Metabolomics,an emerging technology developed after genomics and proteomics,is an important part of systems biology.This study aims to explore the applica-tions of metabolomics in the fields of environment and health.Sample pretreatment technology and detection methods for separating and enriching gallium were chosen through a literature re-view and group discussion.Students then experimentally analyzed the changes in metabolite content in cells cultured with metal-anticancer gallium drugs,which helped them understand the widespread application of metabolomics in the fields of environment and health.Additional-ly,material characterization was conducted using X-ray photoelectron spectroscopy(XPS)and thermogravimetric analysis(TG),and the gallium metal in metabolites was qualitatively and quantitatively analyzed using inductively coupled plasma mass spectrometry(ICP-MS).During discussions of this experiment,the teacher guided students in using large-scale instruments to solve problems comprehensively,fostering a research-based teaching approach to build a solid foundation for conducting efficient instrument analysis and comprehensive experiments within the classroom in future.关键词
综合性实验/仪器分析/固相微萃取/代谢组学/电感耦合等离子体质谱法Key words
comprehensive experiment/instrument analysis/solid phase microextraction(SPME)/metabonomics/inductively coupled plasma mass spectrometry(ICP-MS)分类
化学化工引用本文复制引用
郑海娇,赵亚明,吴小峰,许海,贾琼..仪器分析综合实验:电感耦合等离子体质谱法测定镓元素[J].色谱,2025,43(3):289-294,6.基金项目
吉林大学实验技术项目(SYXM2024b005).Jilin University Experimental Technology Project(No.SYXM2024b005). （SYXM2024b005）
