生物技术通报2025，Vol.41Issue(2)：51-57,7.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0628
水稻叶片淀粉的分离与理化性质分析
Isolation and Physicochemical Properties Analysis of Starch from Rice Leaves
摘要
Abstract
[Objective]Separating high-quality leaf starch is conducive to studying the physicochemical properties and developmental regulation mechanisms of leaf starch.The separation of rice leaf starch using reported leaf starch extraction methods is complicated,requires high operational requirements,and the purity of the separated rice leaf starch is not high.Therefore,it is necessary to establish a method suitable for extracting starch from rice leaves.[Method]We optimized and obtained a simple method for separating and purifying rice leaf starch,which mainly includes nine steps:leaf grinding,cell splitting,starch release,filtration,impurity removal,standing sedimentation,starch washing,ethanol dehydration and drying preservation.The improvement of this method lies in using standing sedimentation to replace the centrifugal collection of starch in Arabidopsis leaf starch extraction method,which can effectively separate rice leaf starch through 3 times of suspension settling.[Result]Using this method to separate and purify starch from three types of rice leaves,the purity of starch in the precipitation reached 87%.Compared with the polygonal shape of endosperm starch granules,the leaf starch granules were flat and long ellipsoidal,whose size were significantly smaller than that of endosperm starch granules in rice.The thermodynamic property assay showed that the gelatinization onset temperature,gelatinization peak temperature and gelatinization completion temperature of leaf starch were significantly higher than those of endosperm starch,while the gelatinization enthalpy was lower than that of endosperm starch in rice.[Conclusion]The optimized method for separating and purifying rice leaf starch is simple and environment-friendly,by which the high-purity leaf starch is obtained for the study of starch morphology and physicochemical properties.关键词
水稻/叶片淀粉/淀粉粒/淀粉提纯/淀粉形态Key words
rice/leaf starch/starch granule/starch purification/starch morphology引用本文复制引用
方慧敏,顾艺枢,张晶,张龙..水稻叶片淀粉的分离与理化性质分析[J].生物技术通报,2025,41(2):51-57,7.基金项目
国家自然科学基金项目(32001450),江苏省自然科学基金项目(BK20221283),扬州大学大学生创新创业训练计划项目(C202311117015Y),江苏高校"青蓝工程"项目(苏教师函[2022]2号),扬州大学"青蓝工程"项目 （32001450）
