生物技术通报2025，Vol.41Issue(2)：58-64,7.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0729
VIGS技术在棉花全生长周期中的应用拓展研究
Extended Research on the Application of VIGS Technology in the Whole Growth Cycle of Cotton
摘要
Abstract
[Objective]To break through the time limit bottleneck that cotton VIGS technology can only be applied in cotton cotyledon stage,and make up for the application deficiency of VIGS technology in cotton reproductive growth stage.[Method]Using Nongdamian 8 as the material and GhCLA1 as the verification gene,4-day-old cotton seedlings'stem(cotyledon folded)were injected,7-day-old cotton seedlings cotyledon(cotyledon fully unfolded)were injected,also flowering and bolling stages cotton stem and base of new fruit branches and new leaves were injected with VIGS respectively.Phenotype observation and RT-PCR were used to verify the silencing efficiency of target genes and photobleaching duration.[Result]The leaf photobleaching rate of 4-day-old cotton seedlings was 80%by VIGS injected to stems,that of 7-day-old cotton seedlings was 100%by VIGS injected to cotyledon,and that of flowering stage and bolling stage cotton was 90%and 68.75%by VIGS injection at the base of new fruit branches,respectively.[Conclusion]A stable VIGS application technology system for the whole growth cycle of cotton is obtained.关键词
基因沉默/棉花/子叶/茎/生长期Key words
VIGS/cotton/cotyledon/stem/growing period引用本文复制引用
丁若羲,豆硕,安叶芝,孔文慧,郭文静,张冬梅,王省芬,马峙英,吴立柱..VIGS技术在棉花全生长周期中的应用拓展研究[J].生物技术通报,2025,41(2):58-64,7.基金项目
国家自然科学基金项目(32072109),河北省中央引导地方科技发展资金项目(236Z2908G) （32072109）
