生物技术通报2025，Vol.41Issue(2)：85-96,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0718
小麦蛋白质二硫键异构酶基因家族的鉴定与表达
Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat
摘要
Abstract
[Objective]The objective of this study is to perform the genome-wide identification of the wheat PDIL(Protein Disulfide Isomerase)gene family members,and to analyze their expression patterns in different organs and developmental stages of wheat,as well as in response to biotic and abiotic stress,which lays the foundation for clarifying the function of wheat PDIL gene family.[Method]Using protein sequences of PDIL gene family of Arabidopsis thaliana and Gramineae crop barley as bait,genome-wide identification of wheat PDIL family genes was conducted in the genome database.Then the physicochemical properties,phylogenetics,chromosome position and gene structure were analyzed using bioinformatics methods.And the expression pattern of PDILs genes in wheat growth and development,biotic and abiotic adversity were analyzed.[Result]The 31 wheat PDIL gene family members were identified as hydrophilic proteins,of which 26 were stable proteins.Subcellular localization prediction indicated that wheat PDIL proteins were mainly distributed in the cytoplasm and chloroplasts,as well as in the endoplasmic reticulum and nucleus.The wheat PDIL gene family could be divided into 7 evolutionary branches,and the gene structure and conserved motif of the same branch were similar or identical,and they were not uniformly distributed on 19 chromosomes.The promoters of wheat PDIL gene family members contained multiple cis-acting elements that responded to stress and associated with five types of plant hormones,including salicylic acid(SA),methyl jasmonate(MeJA),gibberellin(GA),abscisic acid(ABA),and auxin(IAA),and a few genes also contained elements related to circadian rhythm control and seed trait regulation.Transcriptome data showed that TaPDIL4-1A,TaPDIL4-1B,and TaPDIL4-1D genes had high expressions in tissues at different growth stages.Transcriptome analysis and RT-PCR results showed that the relative expression of TaPDIL4-1B increased after infection with Fusarium graminearum,and the expression of TaPDIL1-4A gene increased under drought stress.[Conclusion]The 31 members of the wheat PDIL gene family have conservation during evolution,not only participating in wheat quality formation,but also widely involved in biotic and abiotic stress regulation.Among them,the TaPDIL4-1B gene might be involved in the regulation of wheat FHB resistance,while the TaPDIL1-4A gene might be involved in the response to wheat drought stress.关键词
小麦/PDIL基因家族/表达分析/生物胁迫/非生物胁迫Key words
wheat/PDIL gene family/expression analysis/biotic stress/abiotic stress引用本文复制引用
葛仕杰,刘怡德,张华东,宁强,朱展望,王书平,刘易科..小麦蛋白质二硫键异构酶基因家族的鉴定与表达[J].生物技术通报,2025,41(2):85-96,12.基金项目
国家自然科学基金项目(32272170),武汉市知识创新专项(2022020801010341) （32272170）
