生物技术通报2025，Vol.41Issue(2)：107-118,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0688
过表达SlWRKY41提高番茄幼苗抗旱性
Overexpression of SlWRKY41 Improves the Tolerance of Tomato Seedlings to Drought
摘要
Abstract
[Objective]To explore the role of SlWRKY41 transcription factor in response to drought stress in tomato,and to provide theoretical value and important basis for the enrichment of tomato germplasm resources and the selection and breeding of drought-resistant varieties of tomato.[Method]The experiment was conducted with wild-type tomato'Ailsa Craig'as the material,and its cDNA was used as the template to clone the SlWRKY41 gene to construct the OE-SlWRKY41 transgenic tomato lines,and the wild-type and OE-SlWRKY41 transgenic tomato lines were subjected to drought stress treatments,and the phenotypic observations and related physiological and biochemical indexes were determined at the day 4 and 8 of the treatment.[Result]Compared with wild-type plants,after 8 d of drought stress,OE-SlWRKY41 transgenic plants had curled leaves with less wilting and significantly lower levels of membrane lipid peroxidation.Stomatal conductance and transpiration rates were significantly lower,and the net photosynthetic rate was significantly higher.The root morphology indexes showed an overall upward trend,and the leaf relative water content,antioxidant enzyme activities and osmotic regulating substances(soluble sugars and proline content)increased significantly,and relative conductivity decreased significantly.The stress response genes(SlSOD,SlPOD and SlP5CS1)in the plants overexpressing SlWRKY41 were significantly up-regulated.[Conclusion]SlWRKY41 respond to drought,and the overexpression of SlWRKY41 can improve the tolerance of tomato seedlings to drought stress.关键词
番茄/转录因子/SlWRKY41/干旱胁迫/光合/抗氧化系统/渗透调节Key words
tomato/transcription factors/SlWRKY41/drought stress/photosynthesis/antioxidant system/osmoregulation引用本文复制引用
刘洁,王飞,陶婷,张玉静,陈浩婷,张瑞星,石玉,张毅..过表达SlWRKY41提高番茄幼苗抗旱性[J].生物技术通报,2025,41(2):107-118,12.基金项目
山西省基础研究计划(20210302123366,202203021211263) （20210302123366,202203021211263）
