生物技术通报2025，Vol.41Issue(2)：150-162,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0689
糜子PmDEP1和PmEP3基因的克隆与表达特征分析
Cloning and Expression Characteristics Analysis of Millet Genes PmDEP1 and PmEP3
摘要
Abstract
[Objective]Panicle type is an important factor affecting crop yield and mechanized harvesting.DEP1(Dense Erect Panicles 1)and EP3(Erect Panicles 3)genes are key ones controlling spike type formation.This study aims to explore the structure and expression characteristics of PmDEP1 and PmEP3 ones in proso millet(Panicum miliaceum L.).[Method]PmDEP1 and PmEP3 genes were cloned and subjected to bioinformatics analysis using the scattered ear type millet varieties,broomstick millet(ZM),dense ear type millet M278,and lateral ear type millet M350 as materials.RT-qPCR was used to detect the expression patterns of PmDEP1 and PmEP3 genes in different ear types of proso millet.[Result]Sequence result analysis shows that,the full-length cDNA of PmDEP1 is 1 044 bp,encoding 347 amino acids.The protein domain is predicted to be PAT1.The secondary structure is dominated by random coils.The tertiary structure is highly similar with that of rice DEP1 protein.It is predicted to be located in the nucleus and has high homology with switchgrass.It has 28 phosphorylation sites and does not have transmembrane structure and signal peptide.The full-length cDNA of PmEP3 is 1 215 bp,encoding 358 amino acids.The encoded protein belongs to the F-box family.The secondary structure is dominated by random coils and extended chains.The tertiary structure is highly similar with that of rice Os02g0260200 protein.It is predicted to be located in the cytoplasm.It has high homology with switchgrass,does not contain signal peptides,has 32 phosphorylation sites,and has a small amount of transmembrane structure.The RT-PCR results shows that PmDEP1 and PmEP3 genes are expressed in different parts of the plant at different stages.PmDEP1 gene is mainly expressed in the roots at the jointing stage,with the highest expression in the leaves of broomstick millet and M278 at the heading stage,and in the stems of M350.The expressions of PmEP3 gene is the highest in the leaves at the jointing stage,in the leaves of broomstick millet and M278 at the heading stage,and in the ears of M350.[Conclusion]PmDEP1 and PmEP3 genes are spike type genes that may be involved in regulating the formation of proso millet spike types.关键词
糜子/穗型基因/PmDEP1/PmEP3/基因克隆/表达分析Key words
Panicum miliaceum L./spike type gene/PmDEP1/PmEP3/gene cloning/expression analysis引用本文复制引用
许圆梦,曹晓宁,毛娇,王梦瑶,王数,任江陵,刘宇涵,刘思辰,乔治军,王瑞云..糜子PmDEP1和PmEP3基因的克隆与表达特征分析[J].生物技术通报,2025,41(2):150-162,13.基金项目
山西省重点研发项目(2022ZDYF110),中央引导地方科技发展资金(YDZJSX2022A044),谷子高粱产业技术体系谷子糜子生理岗位(CARS-06-14.5-A16),山西省现代农业杂粮产业技术体系(2024CYJSTX03-23) （2022ZDYF110）
