生物技术通报2025，Vol.41Issue(2)：163-174,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0500
响应NO的藜麦CHX基因家族在盐碱胁迫下的表达模式
Expression Patterns of CHX Gene Family in Quinoa in Response to NO under Saline-alkali Stress
摘要
Abstract
[Objective]CHX(cation/H+exchanger)gene family is a unique monovalent cationic transporter and plays an important role in plant growth and development and response to salt-alkali stress.The CHX gene family members were identified from the whole genome of quinoa,and the expression characteristics of related genes were analyzed to provide support for further research on CHX gene function.[Method]Bioinformatics methods were used to identify the CHX gene family members from quinoa genome,and to analyze their physicochemical properties,phylogenetic relationships,chromosome localization,gene replication,gene structure and promoter cis-acting elements.Transcriptome data combined with RT-qPCR were used to analyze the expression patterns of CHX family genes in different organs under mixed saline-alkali stress and exogenous NO treatment.Transient transformation of tobacco was used to detect the subcellular localization of target protein.[Result]A total of 51 CHX genes were identified in quinoa genome,and the CHX family was divided into 5 subfamilies by phylogenetic analysis.Fragment replication was the primary reason for the evolution of the CHX gene family,which has undergone intense purification selection.Based on the transcriptomic analysis,RT-qPCR analysis of 10 CHX genes under salt-alkali stress and NO treatment showed that the other 8 genes were all in response to salt-alkali stress in stems and roots and were positively regulated by exogenous NO except CqCHX-10 and CqCHX-25 were not expressed.Further subcellular localization of CqCHX-17 protein showed that the gene was localized in the nucleus and chloroplast.[Conclusion]Fifty-one CHX family members are identified from quinoa genome.Different members show different expression patterns in different organizations.CHX gene family members respond positively to saline-alkali stress and exogenous NO.It is found that CqCHX-17 may be involved in K+transport and pH regulation of chloroplast matrix.关键词
藜麦/CHX/生信分析/表达分析/盐碱胁迫Key words
quinoa/CHX/bioinformatics analysis/expression analysis/saline-alkali stress引用本文复制引用
贾子健,王宝强,陈立飞,王义真,魏小红,赵颖..响应NO的藜麦CHX基因家族在盐碱胁迫下的表达模式[J].生物技术通报,2025,41(2):163-174,12.基金项目
国家自然科学基金项目(32301775),甘肃农业大学省部共建干旱生境作物学国家重点实验室开放基金课题(GSCS-2021-10),甘肃省自然科学基金项目(23JRRA1426) （32301775）
