生物技术通报2025，Vol.41Issue(2)：187-201,15.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0671
基于转录组分析植物激素对洋葱鳞茎膨大发育的调控机制
Regulation Mechanism of Plant Hormones Related to Onion Bulb Enlargement and Development Based on Transcriptome Analysis
摘要
Abstract
[Objective]The comparative transcriptome of bulb tissues at different stages of onion development was analyzed to provide a basis for studying the molecular mechanism of plant hormones in onion(Allium cepa L.)bulb formation.[Method]Onion bulbs at 33,47,61 and 75 d after seedling emergence were collected as experimental materials.ELISA and gas chromatography were used to quantify the contents of auxin,cytokinin,gibberellin3,abscisic acid and ethylene(precursor ACC)during the bulb enlargement and development.Transcriptome sequencing was performed using high-throughput sequencing technology to elucidate the effects of plant hormones on bulb development at molecular level.[Result]A total of 11 814 differentially expressed genes(DEGs)were detected from bulbs tissue at four developmental stages,of which 3 311 were shared DEGs.KEGG enrichment analysis revealed that DEGs were significantly enriched into"plant hormone signal transduction"pathway in different comparison groups,and 36 major DEGs were screened and characterized in this pathway.Combining the r and P values of each module and trait in WGCNA(weighted gene co-expression network analysis)analysis,two key modules were screened out.Through transcription factor prediction,3 028 transcription factor unigenes were found to be co-expressed with 13 plant hormone signaling genes.The functional annotation of transcription factor unigenes were derived from 46 gene families,the most important of which were zinc finger protein(C2C2,C3H),followed by bHLH,NAC and ERF gene families.[Conclusion]The genes related to plant hormone signal transduction pathway involved in onion bulb development is preliminarily revealed by transcriptome analysis.关键词
洋葱/鳞茎膨大/转录组分析/植物激素/转录因子Key words
onion/bulb enlargement/transcriptome analysis/plant hormone/transcription factor引用本文复制引用
李艳伟,杨妍妍,孙亚玲,霍雨猛,王振宝,刘冰江..基于转录组分析植物激素对洋葱鳞茎膨大发育的调控机制[J].生物技术通报,2025,41(2):187-201,15.基金项目
山东省农业科学院农业科技创新工程(CXGC2024F08),财政部和农业农村部:国家现代农业产业技术体系项目(CARS-24-A-10) （CXGC2024F08）
