生物技术通报2025，Vol.41Issue(2)：221-233,13.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0704
激素和非生物胁迫下荷花GH3基因家族的表达分析
Expression Analysis of the GH3 Gene Family in Nelumbo nucifera under Hormonal and Abiotic Stresses
摘要
Abstract
[Objective]To characterize NnGH3 gene family in lotus(Nelumbo nucifera)and analyze their roles in abiotic stress,and to provide key candidate genetic resources for the breeding of new lotus varieties with stress resistance.[Method]Bioinformatics methods were used to identify the family members of acyl amides synthetase(GH3)from the whole genome of N.nucifera,and to conduct the detailed study on the phytochemical properties,chromosomal localization,tertiary structure prediction,systematic evolution,gene structure,collinearity relationships,and expression patterns of GH3 enzymes.RT-qPCR technology was applied to investigate the expression patterns of NnGH3 family genes under various stress conditions including low temperature(4℃),anaerobic conditions(submergence),salt stress(300 mmol/L NaCl),and exogenous hormone treatments(0.1 mmol/L IAA,1 mmol/L JA,and 5 mmol/L SA).[Result]The fourteen NnGH3 family members were identified from the lotus genome,distributed on 5 chromosomes,and named sequentially as NnGH3.1 to NnGH3.14 based on their chromosomal locations.Their molecular weights ranged from 365 to 630 amino acids,theoretical isoelectric points were all less than 7,and acidic proteins were localized in the nucleus and cytoplasm.Cluster analysis revealed two groups,Group I and Group Ⅱ,within the GH3 family,with most members containing 3-4 exons and GH3 superfamily domains.Collinearity analysis indicated that 2 pairs of genes among the 14 lotus GH3 genes,14 pairs with(Arabidopsis thaliana)GH3 genes,and 6 pairs with(Oryza sativa)GH3 genes presented collinear relationships.Promoter cis-acting element analysis showed a lot of light-,stress-,and hormone-responsive elements within this family.Expression analysis demonstrated that NnGH3 genes negatively responded to low temperature stress,with most members positively responded to SA treatments and salt stress,and specifically responded to IAA and JA treatments as well as anaerobic stress.Notably,genes NnGH3.4 and NnGH3.13 from Group Ⅱ,and NnGH3.3 from Group I,had the most significant expression changes across all treatments.[Conclusion]Fourteen NnGH3 family members are identified and divided into two groups,Group Ⅰ and Group Ⅱ,which all contain GH3 superfamily domains and are acidic proteins.NnGH3 family genes are specifically expressed under different exogenous hormone and abiotic stress conditions.关键词
荷花/GH3基因家族/生物信息学/植物激素/非生物胁迫/表达模式Key words
Nelumbo nucifera/GH3 gene family/bioinformatics/phytohormone/abiotic stresses/expression pattern引用本文复制引用
匡健华,程志鹏,赵永晶,杨洁,陈润乔,陈龙清,胡慧贞..激素和非生物胁迫下荷花GH3基因家族的表达分析[J].生物技术通报,2025,41(2):221-233,13.基金项目
国家自然科学基金地区科学基金项目(32360070),云南省重大科技专项计划(202202AE090028),云南省高层次人才引进计划高端外国专家专项(YNQR-GDWG-2020-001),云南省"兴滇英才计划"青年人才专项(YNQR-QNRC-2018-122),云南省农业基础联合面上项目(202101BD070001-124) （32360070）
