生物技术通报2025，Vol.41Issue(2)：248-256,9.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0430
山新杨谷胱甘肽转移酶基因的生物信息学与表达模式分析
Bioinformatics and Expression Pattern Analysis of Glutathione S-transferase in Populus davidiana×P.bolleana
摘要
Abstract
[Objective]To provide a basis for further study of the function of glutathione transferase(GST)gene in woody plants,the expression pattern of GST genes from Populus davidiana×P.bolleana were analyzed.[Method]The GST genes were cloned and analyzed by bioinformatics of Populus davidiana×P.bolleana.The expression pattern of GST genes under phytohormone and abiotic stresses were analyzed by real-time PCR.[Result]Three GST gene members from Populus davidiana×P.bolleana were cloned.The lengths of these genes were 678,660 and 690 bp,encoding amino acids of 225,219 and 229 aa,respectively.Meanwhile,all three proteins have the typical structure of glutathione transferase,and their promoter regions have elements that can respond to biotic or abiotic stresses as well as plant hormones.In addition,the expressions of these three genes were differently induced by exogenous phytohormones or abiotic stresses.[Conclusion]All the three PdbGSTs may respond to abiotic stresses or the induction of exogenous phytohormone,indicating that they may play roles in response to the stresses in poplar.关键词
谷胱甘肽转移酶/生物信息学分析/基因表达模式/山新杨Key words
glutathione transferase/bioinformatics analysis/gene expression pattern/Populus davidiana×P.bolleana引用本文复制引用
黄颖,遇文婧,刘雪峰,刁桂萍..山新杨谷胱甘肽转移酶基因的生物信息学与表达模式分析[J].生物技术通报,2025,41(2):248-256,9.基金项目
中央高校基本科研业务费专项资金(2572022DS07),黑龙江省省属科研院所科研业务费(LKSB2024-2) （2572022DS07）
