生物技术通报2025，Vol.41Issue(2)：321-330,10.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0773
一株烟叶霉变真菌的分离鉴定及其致霉因素研究
Isolation and Identification of a Fungus from Moldy Tobacco Leaf and Study on Its Mold-causing Factors
摘要
Abstract
[Objective]Conducting biological research on mold-causing fungi mold in tobacco leaves is of significantly economic importance to the cigarette industry.[Method]In this study,the plate separation method was employed to isolate and purify mold-causing fungi from tobacco leaves.Sequencing technology and phylogenetic analysis were used to identify the obtained strains,while a back-crossing test was conducted to confirm their pathogenicity.The mold-causing factors of the strains were determined through growth assays,and model simulations were utilized to assess the relevance of mold-causing factors.[Resul]A mold-causing fungus was isolated from the surface of moldy tobacco leaves,and morphological and ITS sequence homology analysis identified the strain as closely related to Penicillium citrinum,and homology was 99.82%.The pathogenicity test confirmed that this strain,under conditions of 90%relative humidity,induced mold growth on tobacco leaves.The analysis of mold-causing factors revealed that the optimal conditions for colony growth and sporulation of this strain were 30℃and a water activity of 0.99.Gompertz model simulation demonstrated that the onset of mold in tobacco leaves was not significantly correlated with the initial number of fungal spores but was strongly influenced by temperature,water activity,and the interaction between these two factors.[Conclusion]It is confirmed that P.citrinum CY-H4 possessed mold-causing ability,and temperature and water activity are identified as the primary factors causing mold on tobacco leaves.关键词
分离鉴定/烟叶霉变/致霉/模型模拟Key words
isolation and identification/tobacco leaf mildew/mold-causing/model simulation引用本文复制引用
项波卡,周钻钻,冯佳卉,夏琛,李奇,陈春..一株烟叶霉变真菌的分离鉴定及其致霉因素研究[J].生物技术通报,2025,41(2):321-330,10.基金项目
浙江中烟工业有限责任公司科技项目(2021330000340287),国家自然科学基金项目(31461143030) （2021330000340287）
