生物技术通报2025，Vol.41Issue(2)：331-342,12.DOI:10.13560/j.cnki.biotech.bull.1985.2024-0090
基于蛋白质组学方法探讨四氯化碳诱导的小鼠急性肝损伤的差异蛋白表达
Exploring the Relative Differential Protein Expression of Carbon Tetrachloride-induced Acute Liver Injury in Mice Based on the Proteomics Method
摘要
Abstract
[Objective]Labker quantitative(TMT)proteomics was used to explore the mechanism of chemical inducer(carbon tetrachloride,CCl4)causing acute liver injury in mice.[Method]Twenty SPF male BALB/C mice were randomly divided into control and induction groups,with 10 mice in each group,induced by intraperitoneal injection for 8 weeks(2 times/week).The degree of liver injury was evaluated by measuring the blood biochemical level and liver histopathological sections.Proteomic analysis was performed by tandem mass spectrometry labeling technology(TMT),including gene Ontology(GO)annotation,KEGG pathways,and protein interaction network analysis of the found differential proteins.[Result]Compared with the control group,blood indicator of aspartate aminotransferase(AST)was significantly higher(P<0.05);alanine aminotransferase(ALT),y-glutamyl transpeptadase(GGT),total biliary acid(TBA)(P<0.01).Moreover,liver histopathological sections showed significant liver injury in the CCl4 induction group.A total of 5 955 proteins were identified using TMT,with 440 increased intensity and 294 decreased,and differential proteins played important roles in protein expression difference,biomarker application and participation in classical signaling pathways(JAK-STAT,PI3K-Akt,Rap 1,GnRH,AGE-RAGE).There was a significant enrichment of differential proteins in classical signaling pathways and a relatively high enrichment.[Conclusion]CCl4 as an ideal chemical inducer for liver injury evaluation,it is involved in several key processes such as immunity,cell division,apoptosis and autophagy,inflammation and tumor formation.It reduces the pathological mechanism of liver injury in multiple directions and targets and provides a solid theoretical basis and perspective for the research and development of liver protection drugs.关键词
蛋白质组学/四氯化碳/肝损伤/小鼠Key words
proteomics/carbon tetrachloride/liver injury/mice引用本文复制引用
黎伟华,吴璟,金学琴,雷艳丽..基于蛋白质组学方法探讨四氯化碳诱导的小鼠急性肝损伤的差异蛋白表达[J].生物技术通报,2025,41(2):331-342,12.基金项目
宁夏自然科学基金项目(2022AAC03202) （2022AAC03202）
