中国海洋大学学报（自然科学版）2025，Vol.55Issue(3)：23-33,11.DOI:10.16441/j.cnki.hdxb.20240028
三种不同芽孢杆菌对凡纳对虾生长、非特异性免疫及抗病力影响的比较研究
A Comparative Study on Effects of Three Bacillus Species on the Growth Performance,Non-Specific Immunity and Disease Resistance of Penaeus vannamei
摘要
Abstract
An experiment was conducted to study the effect of adding three Bacillus species to feed on the growth performance,non-specific immunity and disease resistance of Penaeus vannamei.Shrimp was fed with the basal diet supplemented with Bacillus subtilis BSXE-1601(BD group),B.cereus BCXE-01(CD group)and B.velezensis 26.3(BV group)at a density of 1×109 CFU·kg-1 in the basal diet.The basal diet without bacterial additives was used as the control group.The experiment lasted for 6 weeks.After the feeding trial,a 14 d Vibrio parahaemolyticus challenge experiment was conducted.Results showed that the final weight,specific growth rate and feed efficiency of shrimp in BD and BV were significantly improved as compared to the control(P＜0.05),especially in BV.The activities of peroxidase(POD),superoxide dismutase(SOD),acid phosphatase(ACP)and phenol oxidase(PO)in the serum of shrimp of three treatment groups were significantly higher than those of the control group(P＜0.05).The activities of ACP,AKP and SOD in the serum of BV were significantly higher than those of BD(P＜0.05).Compared with the control,the relative expression level of proPO,LZM,SOD,HSP70,Imd,Toll,Relish,TOR,4E-BP,eIF4E1α and eIF4E2 in the hepatopancreas of shrimp of BD was significantly higher than that of the control(P＜0.05).The relative expression level of proPO、LGBP、Imd、Toll、4E-BP and eIF4E2 in the hepatopancreas of shrimp of CD group was significantly higher than that of the control(P＜0.05).The relative expression level of proPO,LZM,SOD,LGBP,HSP70,Imd,Toll,TOR,4E-BP and eIF4E1α in BV was significantly higher than that of the control(P＜0.05).The relative expression level of SOD,LGBP,Toll and 4E-BP in BV was significantly higher than that of CD and BD(P＜0.05).The challenge test of V.parahaemolyticus revealed that the cumulative mortality of shrimp in three treatment groups was significantly lower than that in the control(P＜0.05).In conclusion,the effects of adding B.subtilis BSXE-1601 and B.velezensis 26.3 in the feed markedly enhanced the growth performance of P.vannamei.Two Bacillus species elicited a superior immune response compared to B.cereus BCXE-01.Three Bacillus species significantly enhanced the resistance against V.parahaemolyticus of P.vannamei.关键词
生长/非特异性免疫/抗病力/副溶血弧菌/芽孢杆菌/凡纳对虾Key words
growth/non-specific immunity/disease resistance/Vibrio parahaemolyticus/Bacillus/Penaeus vannamei分类
水产学引用本文复制引用
田相利,李明静,郭泽阳,罗凯,李长林,马振华..三种不同芽孢杆菌对凡纳对虾生长、非特异性免疫及抗病力影响的比较研究[J].中国海洋大学学报（自然科学版）,2025,55(3):23-33,11.基金项目
国家重点研究发展计划项目(2023YFD2401705,2020YFD0900200)资助Supported by the National Key Research and Development Program of China(2023YFD2401705,2020YFD0900200) （2023YFD2401705,2020YFD0900200）
