中国海洋大学学报（自然科学版）2025，Vol.55Issue(3)：70-82,13.DOI:10.16441/j.cnki.hdxb.20240070
溶解性生物炭对水体中转化介导的抗生素抗性基因水平转移的影响
Effect of DissolvedBiochar on the Horizontal Transfer of Extracellular Antibiotic Resistance Genes by Transformation in Aquatic Environments
摘要
Abstract
The coexistence of extracellular antibiotic resistance genes(eARGs)and dissolved biochar(DBC)in surface water environments has drawn widespread attention.However,the effect of DBC on the transformation-mediated horizontal transferprocess of eARGs in aquatic environment remains un-known.In this study,DBC released from reed(Phragmites australis)and reed bamboo(Arundo donax)biochar at different pyrolysis temperatures(300～600 ℃)was selected to investigate its effect on the transformation of the resistance plasmid pBR322 into Escherichia coli(E.coli)DH5a.The re-sults showed that the effect of DBC on the transformation of pBR322 was promoted by low concentration(1 mg·L-1)and inhibited by high concentration(10 mg·L-1),and the inhibitory effect of DBC in-creased with the rising of pyrolysis temperature.DBC could inactivate the eARGs through disrupting the structure of the pBR322 plasmid.At low concentrations,soluble microbial byproduct-like material,and heavy metals in DBC can induce its SOS responsein recipient bacteria,thereby facilitating their up-take of plasmids.However,at high concentrations(5～10 mg·L-1),DBC activated the cellular stress defense system of the bacteria,decreased cell membrane permeability,and hindered the access of plas-mid to the competent cell.Meanwhile,random forest model(R2=0.627 9,MSE=0.002 67)revealed that fulvic acid,soluble microbial byproduct-like material,pH value and heavy metals in DBC play key roles in the transformation process.In addition,structural equation modelling demonstrated that the re-duction of DNA replication activity is the main factor inhibiting the transformation of eARGs.This study extends our understanding of how dissolved organic matter drives the environmental fate of emerging pollutants ARGs in aquatic environments,providing a scientific basis for the establishment of biochar-based pollution control technologies for ARGs in aquatic environments.关键词
溶解性生物炭/胞外抗生素抗性基因/质粒/水平基因转移/氧化应激Key words
dissolved biochar/extracellular antibiotic resistance gene/plasmid/horizontal gene trans-fer/oxidative stress分类
环境科学引用本文复制引用
刘一帆,马小涵,刘柳青青,华健,楚沉静,郑浩,罗先香,李锋民..溶解性生物炭对水体中转化介导的抗生素抗性基因水平转移的影响[J].中国海洋大学学报（自然科学版）,2025,55(3):70-82,13.基金项目
海南省重点研究与发展计划项目(ZDYF2022SHFZ018) （ZDYF2022SHFZ018）
山东省自然科学基金杰出青年基金(ZR2021JQ13) （ZR2021JQ13）
海南省自然科学基金创新研究团队项目(423CXTD384)资助Supported by the Hainan Provincial Key Research and Development Projects(ZDYF2022SHFZ018) （423CXTD384）
the Natural Science Foundation for Distinguished Young Scholars of Shandong Province(ZR2021JQ13) （ZR2021JQ13）
the Hainan Provincial Natural Science Foundation of China(423CXTD384) （423CXTD384）
