饲料研究2025，Vol.48Issue(1)：14-20,7.DOI:10.13557/j.cnki.issn1002-2813.2025.01.003
胍基乙酸、菌酶复合物对公羊血清生化指标、精液品质及抗氧化指标的影响
Effects of guanidinoaceticacid,bacterial enzyme complex on serum biochemical indexes,semen quality,and antioxidant indexes of ram
摘要
Abstract
The experiment aimed to investigate the effects of adding guanidine acetic acid(GAA),s.cerevisiae and cellulase complex(SCC)to the diet on the serum biochemical indexes,semen quality,and antioxidant indexes of rams.A total of 24 healthy Dorper rams with a body weight of(75.0±2.5)kg were randomly divided into six groups,with four replicates per group and one ram per replicate.Using a 3 × 2 factorial completely randomized trial design was employed,with GAA(0,0.03％,0.06％)× SCC(0,0.01％)to form six groups,namely(G0,G1,G2,G3,G4,and G5 groups).The preliminary period lasted seven days,and the formal period lasted 60 days.The results showed that the 0.06％GAA group significantly increased the total protein(TP),insulin(INS),growth hormone(GH),testosterone(TE)content,and aspartate aminotransferase(AST)activity in the serum(P＜0.05),and significantly decreased glucose(GLU)content(P＜0.05).The 0.01％SCC group significantly increased TP content(P＜0.05),the GAA × SCC had significant interactive effects on TP,albumin(ALB),GH,and TE(P＜0.05).The 0.06％GAA group and the 0.01％SCC group significantly improved ram sperm vitality and membrane integrity(P＜0.05),and significantly reduced sperm deformity rate(P＜0.05),the GAA × SCC had significant interactive effects on semen quality indicators(P＜0.05).The 0.06％GAA group significantly increased the activity of superoxide dismutase(SOD)and total antioxidant capacity(T-AOC)in the seminal plasma and serum(P＜0.05),the 0.01％SCC group significantly increased serum SOD activity,significantly increased seminal plasma and serum T-AOC(P＜0.05),and significantly decreased malondialdehyde(MDA)content in seminal plasma and serum(P＜0.05),the GAA × SCC had significant interactive effects on SOD and glutathione peroxidase(GSH-Px)activity,MDA content,and T-AOC(P＜0.05).The study indicates that the simultaneous addition of 0.06％GAA and 0.01％SCC in the diet can effectively improve the ejaculate volume,semen quality,testosterone content,and protein content,enhance the antioxidant capacity in seminal plasma and serum of rams.关键词
胍基乙酸/酿酒酵母菌和纤维素酶复合物/公羊/精液品质/抗氧化指标Key words
guanidine acetic acid/s.cerevisiae and cellulase complex/rams/semen quality/antioxidant indexes分类
农业科技引用本文复制引用
李秀丽..胍基乙酸、菌酶复合物对公羊血清生化指标、精液品质及抗氧化指标的影响[J].饲料研究,2025,48(1):14-20,7.基金项目
山东省羊产业技术体系(项目编号:SDAIT-10-05) （项目编号:SDAIT-10-05）
山东省高等学校畜禽良种繁育新技术研发中心项目(2022) （2022）
潍坊市科技发展计划(项目编号:2022GX027) （项目编号:2022GX027）
