中国海洋大学学报（自然科学版）2025，Vol.55Issue(3)：83-93,11.DOI:10.16441/j.cnki.hdxb.20240050
生物炭和细菌联合矿化盐渍土中有机氮的效能和机制研究
Study on the Efficiency and Mechanism of Combined Mineralization of Organic Nitrogen in Saline Soil by Biochar and Bacteria
摘要
Abstract
Organic nitrogen in coastal saline soil is difficult to convert and utilize due to high salinity and low C/N,necessitating the exploration of amendments to enhance the efficacy of mineralizing organ-ic nitrogen.Iron-modified biochar(FBC)loaded with Bacillus subtilis(BS)was used to prepare a bio-char-based microbial agents(FBCS)to investigate the synergistic effects of FBC and BS on the biomin-eralization and abiomineralization of organic nitrogen.The results showed that FBC was a good carrier for bacterial immobilization,and the number of adsorbed microorganisms was 2.08 times higher than that of the original biochar(BC).Mineralization experiments revealed that the net mineralization of soil organic nitrogen in the FBC,BS,and FBCS treatments increaseed by 32.07％,83.06％,and 145.67％,respectively,compared to the control group(CK).A significant synergistic interaction was observed between FBC and BS,with a synergy coefficient(E)of 36.57％.In the soil environment with high sa-linity and low C/N,the FBCS treatment adsorbed more Na+,elevated the C/N,and enhanced the min-eralization of organic nitrogen.The mechanism of mineralization of organic nitrogen by FBCS was as follows:FBC immobilizing a large quantity of Bacillus subtilis,stimulating bacterial secretion of nitro-gen mineralizing enzymes,thereby enhancing the rate of organic nitrogen mineralization through biotic processes.Simultaneously,FBCS mitigates the adverse effects of high salinity and low C/N on organic nitrogen mineralization,inducing the generation of free radicals such as·OH,thus promoting abiotic mineralization of organic matter.This research provides a novel approach to facilitate the mineralization process of organic nitrogen in saline soil.关键词
盐渍土/生物炭/枯草芽孢杆菌/有机氮矿化/自由基Key words
saline soil/biochar/Bacillus subtilis/organic nitrogen mineralization/free radicals分类
环境科学引用本文复制引用
寇晗,陈友媛,孙萍,李浩,辛佳..生物炭和细菌联合矿化盐渍土中有机氮的效能和机制研究[J].中国海洋大学学报（自然科学版）,2025,55(3):83-93,11.基金项目
国家自然科学基金项目(42177053)资助Supported by the National Natural Science Foundation of China(42177053) （42177053）
