中国海洋大学学报（自然科学版）2025，Vol.55Issue(3)：145-154,10.DOI:10.16441/j.cnki.hdxb.20230111
钙离子和非肌球蛋白对斑马鱼表皮离子细胞顶端开口的调控作用
Regulatory Effects of Calcium and Non-Myosin on Apical Opening of Zebrafish Epidermal lonocytes
摘要
Abstract
In order to study the factors affecting the size of the apical opening of ionocytes,this study used zebrafish as a model organism to explore the factors by immunofluorescence and drug treatment.The apical opening was usually investigated via electron microscopy,which is relatively laborious.In this study,we reported a simple method to visualize apical opening of ionocytes via antibody staining of Claudin-5,a tight junction protein.We analyzed the apical opening of ionocytes using several small mol-ecule compounds.Our data showed that treatment of zebrafish embryos with calcium inhibitors reduced the size of apical opening in ionocytes.Similarly,incubation with inhibitor of non-muscle myosin Ⅱ also resulted in the decreased size of apical opening.In contrast,treatment with either epinephrine or thyroid hormones had no effect on the apical opening of ionocytes.These results suggested that the apical open-ing of ionocytes is controlled by a calcium dependent regulatory mechanisms which may require the in-volvement of non-muscle myosin Ⅱ.Our data may provide further information for the study of the ho-meostasis and physiological function of ionocytes.关键词
斑马鱼/离子细胞/顶端开口/钙离子/Claudin-5抗体Key words
zebrafish/ionocyte/apical opening/calcium ion/antibody of Claudin-5分类
生物科学引用本文复制引用
韩骐泽,赵呈天,谢海波..钙离子和非肌球蛋白对斑马鱼表皮离子细胞顶端开口的调控作用[J].中国海洋大学学报（自然科学版）,2025,55(3):145-154,10.基金项目
国家自然科学基金项目(32100661)资助Supported by the National Natural Science Foundation of China(32100661) （32100661）
