中国海洋大学学报（自然科学版）2025，Vol.55Issue(3)：169-183,15.DOI:10.16441/j.cnki.hdxb.20230124
驼背鲈幼鱼脑、肝、肌肉组织适应不同盐度的转录组分析
Transcriptome Analysis on the Salinity Adaptation in Brain,Liver and Muscle Tissues of Juvenile Cromileptes altivelis
摘要
Abstract
This study investigated the effects of long-term aquaculture under different salinity condi-tions on gene expression of the juvenile Cromileptes altivelis,the brain,liver,and muscle tissues rared in low salinity sea water were selected for transcriptome analysis to explore the mechanism of salinity stress on fish.Juveniles with body weight of 1.1～1.7 g were rared in natural sea water with salinity of 32 as control and salinity of 18 as low lalinity group,respectively.Then,the brain,liver,and muscle tissues were collected on days 50 and 60,and the transcriptomes were sequenced by Illumina NovaSeq 6000 platform.Screening conditions for differentially expressed genes(DEGs)were set as p-value＜0.05 and|log2(Fold change)|＞1.The results of enrichment analysis showed that the DEGs in brain tissue between the two experimental groups were significantly more than those in liver and muscle tis-sues.The DEGs in brain tissue were mainly involved in biological processes such as cell division,nutri-ent metabolism and cell cycle.The DEGs in liver tissue were mainly involved in carbohydrate metabo-lism,amino acid metabolism,enzyme complex formation and other processes.The DEGs in muscle tis-sue were mainly related to cell growth and development,division,and growth-related signaling path-ways such as MAPK signaling pathway,calcium signaling pathway,Wnt signaling pathway,and insulin signaling pathway.Real-time fluorescence quantitative PCR(qRT-PCR)was used to verify the expres-sion of three DEGs randomly selected from three tissues.The results of this study provide a reference for the future breeding work of Cromileptes altivelis and the molecular mechanism of salinity adaptation.关键词
驼背鲈/盐度/转录组分析/GO富集分析/KEGG富集分析/实时荧光定量PCRKey words
Cromileptes altivelis/salinity/transcriptome analysis/GO enrichment analysis/KEGG enrichment analysis/real-time fluorescence quantitative PCR分类
农业科技引用本文复制引用
李啸圻,孙慧邦,刘宇翔,刘金相,张全启..驼背鲈幼鱼脑、肝、肌肉组织适应不同盐度的转录组分析[J].中国海洋大学学报（自然科学版）,2025,55(3):169-183,15.基金项目
三亚崖州湾科技城管理局科研项目(SKJC-2020-02-009)资助Supported by the Research Program of Sanya Yazhou Bay Science and Technology City(SKJC-2020-02-009) （SKJC-2020-02-009）
